{"created":"2021-03-01T06:16:23.217070+00:00","id":13459,"links":{},"metadata":{"_buckets":{"deposit":"fadd2f4a-db18-49b1-85d2-56be8e8d201a"},"_deposit":{"id":"13459","owners":[],"pid":{"revision_id":0,"type":"depid","value":"13459"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00013459","sets":["1309:1310"]},"author_link":["41033","41034","41035","41036","41037","41038","41039","41040"],"control_number":"13459","item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"パーティクルガンによるタバコ培養細胞への遺伝子導入 : phenylalanine ammonia-lyase(PAL)cDNAの導入と遺伝子発現"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"171","bibliographicPageStart":"165","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Plant tissue culture letters"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The plasmid DNA containing a NPTⅡ as a selectable marker gene and a tobacco PAL cDNA combined with the CaMV 35S promoter and NOS terminator was transformed into tobacco cells by a particle bombardment device based on flowing helium. The most effcient transformation was achieved when the amount of DNA-coated tungsten particle was 0.2㎎ per projectile, the amount of plasmid DNA was 4μg/㎎ of tungsten particles, the helium pressure accelerated was 3㎏/c㎡, and the distance between the sample and the projectile was 15cm. Genetic analysis of kanamycin-resistant transformants obtained　showed that the PAL cDNA construct integrated into the genome of tobacco cells. PAL activity of the transformant increased almost 4-fold and scopoletin content increased more than 2-fold as compared to nontrasformed cells.","subitem_description_type":"Abstract"},{"subitem_description":"ヘリウムガス圧式パーティクルガンにより，タバコ培養細胞のPALcDNAを同細胞へ再導入し，形質転換体を得た．タバコ培養細胞のPALcDNAをカリフラワーモザイクウイルス(CaMV)の35Sプロモーターとノパリン合成酵素(NOS)遺伝子のターミネーターに接続し，ネオマイシンホスホトランスフェラーゼⅡ(NPTⅡ)遺伝子とともにタバコ培養細胞へ導入し，形質転換体をカナマイシン培地で選抜した．また，パーティクルガンによる遺伝子導入の最適条件としては，加速圧力3㎏/c㎡，0.2㎎タングステン粒子/プロジェクタイル，4μg/㎎タングステン粒子，プロジェクタイルからサンプルまでの距離は15㎝であった．そして得られた形質転換体の中には，非形質転換体と比較して，PAL酵素活性で4倍，スコポレチン生成量で2倍以上のものが確認された．","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Plant tissue culture letters. 12(2):165-171 (1995)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Taguchi, G","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"},{"subitem_link_text":"Shimosaka, M","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本植物細胞分子生物学会"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.5511/plantbiotechnology1984.12.165","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0289-5773","subitem_source_identifier_type":"PISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10050931","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nagai,  N"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagisawa,  K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizuno,  K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imura,  H"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taguchi,  G"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosaka,  M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata,  D"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okazaki,  M"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"pb12_165.pdf","filesize":[{"value":"885.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"pb12_165.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/13459/files/pb12_165.pdf"},"version_id":"8f1fc976-e784-4c32-9898-5cd9537e15e6"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Transformation of Tobacco Cultured Cell by Particle Bombardment : Expression of Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transformation of Tobacco Cultured Cell by Particle Bombardment : Expression of Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Cells.","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-02-12"},"publish_date":"2010-02-12","publish_status":"0","recid":"13459","relation_version_is_last":true,"title":["Transformation of Tobacco Cultured Cell by Particle Bombardment : Expression of Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:17:46.768921+00:00"}