{"created":"2021-03-01T06:17:25.349733+00:00","id":14470,"links":{},"metadata":{"_buckets":{"deposit":"315cecd9-928a-4503-9ddd-b930ef032351"},"_deposit":{"id":"14470","owners":[],"pid":{"revision_id":0,"type":"depid","value":"14470"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00014470","sets":["1309:1314:1458:1461"]},"author_link":["43584"],"item_11_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"天然繊維の高機能化と応用"}]},"item_11_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"53","bibliographicPageStart":"53","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"先進ファイバー工学研究教育拠点研究成果報告書"}]}]},"item_11_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The Bacillus subtilis CwlC is the cell wall lytic N-acetylmuramoyl-l-alanine amidases in the CwlB (LytC) family that contains a homologous catalytic domain. The enzymes are thought to play an important role in mother-cell lysis in sporulation. The CwlC consists of a N-terminal catalytic domain and a tandem repeat (repeat-1 :184-219 and repeat-2:220-254) in the C-terminal region. Biochemical analysis has shown that the C-terminal tandem repeat, named as CwlCr, can bind to the B. subtilis peptidoglycan (unpublished). We tried to determine the structure Of CwlCr for understanding a peptidoglycan binding mechanism. Using standard multi-dimensional hetero nuclear NMR methods, we completed the main-chain and side-chain resonance assignments, and collected distance restraints and dihedral angle nestraints. Furthermore, unambiguous 26 hydrogen bond restraints were obtained from HNCO(h3JNC) experiment. Structure calculation waspeqormed using CYANA, and a low resolution structure was obtained so far. Intriguingly, the each repeat adopted b a b structure making a b -sheet between repeat1 and repeat2. Thus, it was likely that both repeat-1 and repeat-2 were required for CwlCr folding. The refinement process of structure Calculation and mutation analyses are under way. We will discuss the interaction of CwlCr with peptidoglycan in detail based on an NMR titration experiment.","subitem_description_type":"Abstract"}]},"item_11_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_11_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"先進ファイバー工学研究教育拠点研究成果報告書 11: 53-53(2005)","subitem_description_type":"Other"}]},"item_11_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"志田, 敏夫","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ONTmgNAN.html"}]},"item_11_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州大学繊維学部"}]},"item_11_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1200368X","subitem_source_identifier_type":"NCID"}]},"item_1627890728808":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"志田,  敏夫"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"AFSTT11-211.pdf","filesize":[{"value":"468.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AFSTT11-211.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/14470/files/AFSTT11-211.pdf"},"version_id":"9f09ac9f-931c-48f4-ac12-6d06ec93e706"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"15-2-9 : 微生物酵素によって合成される繊維状高分子に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"15-2-9 : 微生物酵素によって合成される繊維状高分子に関する研究","subitem_title_language":"ja"}]},"item_type_id":"11","owner":"1","path":["1461"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-12-05"},"publish_date":"2011-12-05","publish_status":"0","recid":"14470","relation_version_is_last":true,"title":["15-2-9 : 微生物酵素によって合成される繊維状高分子に関する研究"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:05:05.478802+00:00"}