{"created":"2021-03-01T06:17:25.413807+00:00","id":14471,"links":{},"metadata":{"_buckets":{"deposit":"f848549a-b7a0-48fa-b73f-6404d7404e99"},"_deposit":{"id":"14471","owners":[],"pid":{"revision_id":0,"type":"depid","value":"14471"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00014471","sets":["1309:1314:1458:1461"]},"author_link":["43585","43586"],"item_11_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"バイオテクノロジーを活用した新規繊維生物の作出"}]},"item_11_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"54","bibliographicPageStart":"54","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"先進ファイバー工学研究教育拠点研究成果報告書"}]}]},"item_11_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(1) The first successful achievement of intracytoplasmic sperm injection (ICSI)-mediated transgenesis in the rats was expanded to the another exogenous DNAs with larger chain length (>100 kb) and the different rat strains. The stable integration of the exogenous DNA in the founder transgenic rats was also confirmed from the PCR analyses of next generation offspring. (2) As a storage method of rat spermatozoa prior to the ICSI, the freeze-drying and storage at -200℃ was recommendable, alternative to the conventional Peering without cryoprotective agents and storage at 196℃. Live offspring were produced effectively if the spermatozoa were treated before freeze-drying by ultra-sonication to dissociate the sperm heads from their long tails. (3) Efficiency of producing rat onspring by round spermatid injection (ROSI) was improved when oocytes were pre-treated for induced activation by direct current pulses plus 6-dimethylaminopurine rather than strontium chloride. In vitro production of developmentally competent round spermatids by culturing spermatogonial stem cells with somatic Sertoli cells is being attempted.","subitem_description_type":"Abstract"}]},"item_11_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_11_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"先進ファイバー工学研究教育拠点研究成果報告書 11: 54-54(2005)","subitem_description_type":"Other"}]},"item_11_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"保地, 眞一","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html"},{"subitem_link_text":"木村, 建","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ymLajekV.html"}]},"item_11_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州大学繊維学部"}]},"item_11_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1200368X","subitem_source_identifier_type":"NCID"}]},"item_1627890728808":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"保地,  眞一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木村,  建"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"AFSTT11-212.pdf","filesize":[{"value":"425.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AFSTT11-212.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/14471/files/AFSTT11-212.pdf"},"version_id":"273eb250-1b85-4a19-b27c-3c38e3e9cdf8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"15-2-10 : 顕微授精システムを利用した形質転換動物の作出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"15-2-10 : 顕微授精システムを利用した形質転換動物の作出","subitem_title_language":"ja"}]},"item_type_id":"11","owner":"1","path":["1461"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-12-05"},"publish_date":"2011-12-05","publish_status":"0","recid":"14471","relation_version_is_last":true,"title":["15-2-10 : 顕微授精システムを利用した形質転換動物の作出"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:05:04.875231+00:00"}