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Lcc I showed the decolorization against phthalocyanine and dimo dyes, and Lcc II decolorized monoazo dye. During culture, Lcc II was produced at the early stage and Lcc I was produced at the later stage. Each Lcc was pur\u0027jied and the characterization was investigated\uff65 Their molecular masses were estimated to be 60 kDa (Lcc I) and 58 kDa (Lcc II), and the optimum pH commonly showed at 3\uff655\uff65 Lcc II showed stabilityat alkaline pH and high temperature more than Lcc I\uff65 On the other hand\uff65 the partial CDNA sequences of eight Lcc isozymes were identljied by RFLP combined with RT-PCR\uff65 They were divided into two groups, and all showed high similarityto the other Lcc produced by wood-rot jhngi\uff65 These sequences might be Lcc I, Lcc II and contain a more available en\u30b0mefor degradation ofmanypollutants\uff65pleurotus salmoneostramineus produced two laccase (Lcc) isozymes showing different specificity for dye decolorization. Lcc I showed the decolorization against phthalocyanine and diazo dyes, and Lcc II decolorized monoazo dye. During culture, Lcc II was produced at the early stage and Lcc I was produced at the later stage. Each Lcc was purified and the characterization was investigated. Their molecular masses were estimated to be 60 kDa (Lcc I) and 58 kDa (Lcc II), and the optimum pH commonly showed at 3.5. Lcc II showed stability at alkaline pH and high temperature more than Lcc I. On the other hand, the partial cDNA sequences of eight Lcc isozymes were identified by RFLP combined with RT-PCR. They were divided into two groups, and all showed high similarity to the other Lcc produced by wood-rot fungi. These sequences might be Lcc I, Lcc II and contain a more available enzyme for degradation of many pollutants.", "subitem_description_type": "Abstract"}]}, "item_11_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_11_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u5148\u9032\u30d5\u30a1\u30a4\u30d0\u30fc\u5de5\u5b66\u7814\u7a76\u6559\u80b2\u62e0\u70b9\u7814\u7a76\u6210\u679c\u5831\u544a\u66f8 11: 58-58(2005)", "subitem_description_type": "Other"}]}, "item_11_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "\u91ce\u5d0e, \u529f\u4e00", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jpyFbpkh.html"}, {"subitem_link_text": "\u5929\u91ce, \u826f\u5f66", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.gVTVZVkh.html"}]}, "item_11_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66\u7e4a\u7dad\u5b66\u90e8"}]}, "item_11_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_11_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA1200368X", "subitem_source_identifier_type": "NCID"}]}, "item_11_text_66": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "024"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u91ce\u5d0e,  \u529f\u4e00"}], "nameIdentifiers": [{"nameIdentifier": "43593", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5929\u91ce,  \u826f\u5f66"}], "nameIdentifiers": [{"nameIdentifier": "43594", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u795e\u7530,  \u9df9\u4e45"}], "nameIdentifiers": [{"nameIdentifier": "43595", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AFSTT11-215.pdf", "filesize": [{"value": "204.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 204500.0, "url": {"label": "AFSTT11-215.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/14474/files/AFSTT11-215.pdf"}, "version_id": "32533287-383e-4558-87cb-77824ebf90a1"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "15-2-13 : \u96e3\u5206\u89e3\u6027\u7269\u8cea\u5206\u89e3\u306b\u5411\u3051\u305f\u9175\u7d20\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u69cb\u7bc9 : \u67d3\u8272\u5264\u8131\u8272\u9175\u7d20\u306e\u958b\u767a", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "15-2-13 : \u96e3\u5206\u89e3\u6027\u7269\u8cea\u5206\u89e3\u306b\u5411\u3051\u305f\u9175\u7d20\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u69cb\u7bc9 : \u67d3\u8272\u5264\u8131\u8272\u9175\u7d20\u306e\u958b\u767a"}]}, "item_type_id": "11", "owner": "1", "path": ["1309/1314/1458/1461"], "permalink_uri": "http://hdl.handle.net/10091/13402", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2011-12-05"}, "publish_date": "2011-12-05", "publish_status": "0", "recid": "14474", "relation": {}, "relation_version_is_last": true, "title": ["15-2-13 : \u96e3\u5206\u89e3\u6027\u7269\u8cea\u5206\u89e3\u306b\u5411\u3051\u305f\u9175\u7d20\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u69cb\u7bc9 : \u67d3\u8272\u5264\u8131\u8272\u9175\u7d20\u306e\u958b\u767a"], "weko_shared_id": null}