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  1. 080 繊維学部
  2. 0802 紀要・刊行物
  3. 08030 先端繊維技術科学に関する研究報告
  4. Vol. 11

15-2-13 : 難分解性物質分解に向けた酵素ライブラリーの構築 : 染色剤脱色酵素の開発

http://hdl.handle.net/10091/13402
99d2b946-6007-4308-a018-77b52d298d6d
名前 / ファイル ライセンス アクション
AFSTT11-215.pdf AFSTT11-215.pdf (204.5 kB)
Item type 研究報告書 / Research Paper(1)
公開日 2011-12-05
タイトル
言語 ja
タイトル 15-2-13 : 難分解性物質分解に向けた酵素ライブラリーの構築 : 染色剤脱色酵素の開発
言語
言語 jpn
資源タイプ
資源 http://purl.org/coar/resource_type/c_18ws
タイプ research report
その他(別言語等)のタイトル
その他のタイトル バイオテクノロジーを活用した新規繊維生物の作出
著者 野崎, 功一

× 野崎, 功一

WEKO 43593

野崎, 功一

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天野, 良彦

× 天野, 良彦

WEKO 43594

天野, 良彦

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神田, 鷹久

× 神田, 鷹久

WEKO 43595

神田, 鷹久

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信州大学研究者総覧へのリンク
氏名 野崎, 功一
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jpyFbpkh.html
信州大学研究者総覧へのリンク
氏名 天野, 良彦
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.gVTVZVkh.html
出版者
出版者 信州大学繊維学部
引用
内容記述タイプ Other
内容記述 先進ファイバー工学研究教育拠点研究成果報告書 11: 58-58(2005)
書誌情報 先進ファイバー工学研究教育拠点研究成果報告書

巻 11, p. 58-58, 発行日 2005-03-31
抄録
内容記述タイプ Abstract
内容記述 pleurotus salmoneostramineus produced two laccase (Lcc) isozymes showing dlHerent specljicityfor dye decolorization. Lcc I showed the decolorization against phthalocyanine and dimo dyes, and Lcc II decolorized monoazo dye. During culture, Lcc II was produced at the early stage and Lcc I was produced at the later stage. Each Lcc was pur'jied and the characterization was investigated・ Their molecular masses were estimated to be 60 kDa (Lcc I) and 58 kDa (Lcc II), and the optimum pH commonly showed at 3・5・ Lcc II showed stabilityat alkaline pH and high temperature more than Lcc I・ On the other hand・ the partial CDNA sequences of eight Lcc isozymes were identljied by RFLP combined with RT-PCR・ They were divided into two groups, and all showed high similarityto the other Lcc produced by wood-rot jhngi・ These sequences might be Lcc I, Lcc II and contain a more available enグmefor degradation ofmanypollutants・pleurotus salmoneostramineus produced two laccase (Lcc) isozymes showing different specificity for dye decolorization. Lcc I showed the decolorization against phthalocyanine and diazo dyes, and Lcc II decolorized monoazo dye. During culture, Lcc II was produced at the early stage and Lcc I was produced at the later stage. Each Lcc was purified and the characterization was investigated. Their molecular masses were estimated to be 60 kDa (Lcc I) and 58 kDa (Lcc II), and the optimum pH commonly showed at 3.5. Lcc II showed stability at alkaline pH and high temperature more than Lcc I. On the other hand, the partial cDNA sequences of eight Lcc isozymes were identified by RFLP combined with RT-PCR. They were divided into two groups, and all showed high similarity to the other Lcc produced by wood-rot fungi. These sequences might be Lcc I, Lcc II and contain a more available enzyme for degradation of many pollutants.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA1200368X
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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