@techreport{oai:soar-ir.repo.nii.ac.jp:00014474,
 author = {野崎,  功一 and 天野,  良彦 and 神田,  鷹久},
 month = {Mar},
 note = {pleurotus salmoneostramineus produced two laccase (Lcc) isozymes showing dlHerent specljicityfor dye decolorization. Lcc I showed the decolorization against phthalocyanine and dimo dyes, and Lcc II decolorized monoazo dye. During culture, Lcc II was produced at the early stage and Lcc I was produced at the later stage. Each Lcc was pur'jied and the characterization was investigated･ Their molecular masses were estimated to be 60 kDa (Lcc I) and 58 kDa (Lcc II), and the optimum pH commonly showed at 3･5･ Lcc II showed stabilityat alkaline pH and high temperature more than Lcc I･ On the other hand･ the partial CDNA sequences of eight Lcc isozymes were identljied by RFLP combined with RT-PCR･ They were divided into two groups, and all showed high similarityto the other Lcc produced by wood-rot jhngi･ These sequences might be Lcc I, Lcc II and contain a more available enグmefor degradation ofmanypollutants･pleurotus salmoneostramineus produced two laccase (Lcc) isozymes showing different specificity for dye decolorization. Lcc I showed the decolorization against phthalocyanine and diazo dyes, and Lcc II decolorized monoazo dye. During culture, Lcc II was produced at the early stage and Lcc I was produced at the later stage. Each Lcc was purified and the characterization was investigated. Their molecular masses were estimated to be 60 kDa (Lcc I) and 58 kDa (Lcc II), and the optimum pH commonly showed at 3.5. Lcc II showed stability at alkaline pH and high temperature more than Lcc I. On the other hand, the partial cDNA sequences of eight Lcc isozymes were identified by RFLP combined with RT-PCR. They were divided into two groups, and all showed high similarity to the other Lcc produced by wood-rot fungi. These sequences might be Lcc I, Lcc II and contain a more available enzyme for degradation of many pollutants., Article, 先進ファイバー工学研究教育拠点研究成果報告書 11: 58-58(2005)},
 title = {15-2-13 : 難分解性物質分解に向けた酵素ライブラリーの構築 : 染色剤脱色酵素の開発},
 year = {2005}
}