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First of all, we demonstrated here that a novel yvcE (cwl0) gene encodes a vegetative cell wall hydrolase classified into the DL-endopeptidase family. The C-terminal truncated protein, C-Cwl0-6His, retained cell wall hydrolase activity, and the substrate bond specificity indicated that it is a DL-endopeptidase in vitro. CwlO was secreted in culture medium during the vegetative growth phase. The disruption of CwlO, however did not affect cell growth, morphology, and motility. Second we investigated that the LysM domain of CwlE specifically recognized naked peptidoglycan in cell wall. In addition, immunofluorescence microscopy of CwlE-3xFLAG with several mutants in the teichoic acid biosynthesis pathway strongly suggested that peptidoglycan modification by major and minor wall teichoic acids might mainly occur in the cylindrical part of a rod-shaped cell.", "subitem_description_type": "Abstract"}]}, "item_11_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_11_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u5148\u9032\u30d5\u30a1\u30a4\u30d0\u30fc\u5de5\u5b66\u7814\u7a76\u6559\u80b2\u62e0\u70b9\u7814\u7a76\u6210\u679c\u5831\u544a\u66f8 11: 59-59(2005)", "subitem_description_type": "Other"}]}, "item_11_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "\u5c71\u672c, \u535a\u898f", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.uhDNjekV.html"}]}, "item_11_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66\u7e4a\u7dad\u5b66\u90e8"}]}, "item_11_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_11_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA1200368X", "subitem_source_identifier_type": "NCID"}]}, "item_11_text_66": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "025"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u95a2\u53e3,  \u9806\u4e00"}], "nameIdentifiers": [{"nameIdentifier": "43596", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5c71\u672c,  \u535a\u898f"}], "nameIdentifiers": [{"nameIdentifier": "43597", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AFSTT11-216.pdf", "filesize": [{"value": "508.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 508500.0, "url": {"label": "AFSTT11-216.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/14475/files/AFSTT11-216.pdf"}, "version_id": "167e147c-f1e9-467a-bbbd-2f6dae31abff"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "15-2-14 : \u7d30\u80de\u58c1\u5408\u6210\uff65\u4fee\u98fe\uff65\u5206\u89e3\u6a5f\u69cb\u306e\u7814\u7a76", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "15-2-14 : \u7d30\u80de\u58c1\u5408\u6210\uff65\u4fee\u98fe\uff65\u5206\u89e3\u6a5f\u69cb\u306e\u7814\u7a76"}]}, "item_type_id": "11", "owner": "1", "path": ["1309/1314/1458/1461"], "permalink_uri": "http://hdl.handle.net/10091/13444", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2011-12-05"}, "publish_date": "2011-12-05", "publish_status": "0", "recid": "14475", "relation": {}, "relation_version_is_last": true, "title": ["15-2-14 : \u7d30\u80de\u58c1\u5408\u6210\uff65\u4fee\u98fe\uff65\u5206\u89e3\u6a5f\u69cb\u306e\u7814\u7a76"], "weko_shared_id": null}