@techreport{oai:soar-ir.repo.nii.ac.jp:00014487, author = {藤井, 敏弘 and 平井, 利博}, month = {Mar}, note = {Isolation of biopolymers, tissues, and cells from the host body system is necessary to protect the implanted polymers from protease and immune systems. Encapsulation technology is thought to be an effective for solving these problems, and further can be used in bioreactor and biosensor fields. Muscle proteins such as myosin, action, and actomyosin were entrapped in gellan/chitosan polyion complex (PIC) capsules using a simple procedure. The incorporation of muscle proteins was examined by ATP hydrolysis and electrophoresis. The recoveries of myosin and actomyosin ATPase activities were approximately 30% and 35%, respectively, when the capsules were prepared by dropping a mixture solution of the proteins and gellan into a chitosan solution. The activation of myosin Mg2+-ATPase activity, by actin was also observed after their incorporation into the capsules. Myosin in the capsules mainly consisted of heavy chain and light chains without remarkable cleavage. These results suggest that this system will be an excellent model for contractile apparatus., Article, 先進ファイバー工学研究教育拠点研究成果報告書 11: 78-78(2005)}, title = {15-3-10 : 細胞内繊維構造体の分子集合を利用したマイクロマシーンの創出}, year = {2005} }