@techreport{oai:soar-ir.repo.nii.ac.jp:00014583,
 author = {志田,  敏夫},
 month = {Mar},
 note = {DNA fragment (tgl gene) for construction of Tgl (transglutaminase) expression plasmid was amplyfied by PCR (polymerase chain reaction) method using B. subtilis 168 chromosal DNA as atemplate. To obtain the His-tagged transglutaminase of B. subtilis, the fragment was cloned into the expression plasmid pQE30. Competent E. coli KP3998 was transformed with the resulting plasmid pQE-Tgl. The His-tagged Tgl protein was purified with His Trap column., Article, 先進ファイバー工学研究教育拠点研究成果報告書 10: 54-54(2004)},
 title = {15-2-9 :微生物酵素のよって合成される繊維状高分子に関する研究},
 year = {2004}
}