@techreport{oai:soar-ir.repo.nii.ac.jp:00014586,
 author = {野末,  雅之 and 斉藤,  英毅 and 野川,  優洋 and 小島,  峯雄},
 month = {Mar},
 note = {Molecular breeding of crop plants using genetic transformation is a promising approach for crop improvement. Molecular breeding requires a development of a gene transfer method for each crop. We have already developed a simple and efficient in planta transformation method for buckwheat. In this year, we have developed the in planta transformation methods for mulberry and kenaf plants. In the both methods, we used an avirulent mutant (M-21) of A. tumefaciens, which had been isolated by us, for the following reason; in this mutant, tryptophan monooxygenase gene in T-DNA that is involved in auxin biosynthesis is destructed, whereas all other genes including ipt gene for cytokinin biosynthesis are intact. Consequently, transformants produced by this A. tumefaciens were expected to show a dramatically altered phenotype due to hormone imbalance, which is a good indication of transformation. Young mulberry plants in pots were decapitated and the meristem of axillary buds were inoculated with A. tumefaciens. The inoculated mulberry plants were allowed to grow in pots. With kenaf plants, the meristem of either apical or axillary buds were inoculated with A. tumefaciens and grown as mulberry plants. Transformation of both plants is demonstrated by phenotype and PCR detection of transgene using the second generation of transformants, namely the offspring propagated by stem cuttage of mulberry, and T1 plant ofr kenaf The transformants of both plants showed economically good traits; well branched phenotype with mulberry and thicker stem with kenaf., Article, 先進ファイバー工学研究教育拠点研究成果報告書 10: 57-58(2004)},
 title = {15-2-12 :繊維作物の分子育種のための基盤技術の開発},
 year = {2004}
}