{"created":"2021-03-01T06:20:53.996967+00:00","id":17843,"links":{},"metadata":{"_buckets":{"deposit":"36d0ba9d-cd94-4736-9dd9-39838e79ef81"},"_deposit":{"id":"17843","owners":[],"pid":{"revision_id":0,"type":"depid","value":"17843"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00017843","sets":["1221:1222"]},"author_link":["49845","49846","49847","49848","49849"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"746","bibliographicPageStart":"738","bibliographicVolumeNumber":"79","bibliographic_titles":[{"bibliographic_title":"BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The xylanase (Xyn10B) that strongly adsorbs on microcrystalline cellulose was isolated from Driselase. The Xyn10B contains a Carbohydrate-binding module family 1 (CBM1) (IrpCBM(Xyn10B)) at N-terminus. The canonical essential aromatic residues required for cellulose binding were conserved in IrpCBM(Xyn10B); however, its adsorption ability was markedly higher than that typically observed for the CBM1 of an endoglucanase from Trametes hirsuta (ThCBMEG1). An analysis of the CBM-GFP fusion proteins revealed that the binding capacity to cellulose (7.8 mu mol/g) and distribution coefficient (2.0L/mu mol) of IrpCBM(Xyn10B)-GFP were twofold higher than those of ThCBMEG1-GFP (3.4 mu mol/g and 1.2L/mu mol, respectively), used as a reference structure. Besides the canonical aromatic residues (W24-Y50-Y51) of typical CBM1-containing proteins, IrpCBM(Xyn10B) had an additional aromatic residue (Y52). The mutation of Y52 to Ser (IrpCBM(Y52S)-GFP) reduced these adsorption parameters to 4.4 mu mol/g and 1.5L/mu mol, which were similar to those of ThCBMEG1-GFP. These results indicate that Y52 plays a crucial role in strong cellulose binding.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. 79(5):738-746 (2015)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Nozaki, Kouichi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.jpyFbpkh.html"},{"subitem_link_text":"Mizuno, Masahiro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.gFDmbpkh.html"},{"subitem_link_text":"Amano, Yoshihiko","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.gVTVZVkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000354463700008"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"TAYLOR & FRANCIS LTD"},{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"25560084"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25560084","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1080/09168451.2014.996203"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1080/09168451.2014.996203","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is an Accepted Manuscript of an article published by Taylor & Francis in 'Biochemistry & Molecular Biology' on 03 Jan 2015, available online: http://www.tandfonline.com/10.1080/09168451.2014.996203 ."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishijima,  Hiroto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nozaki,  Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizuno,  Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arai,  Tsutomu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Amano,  Yoshihiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-03"}],"displaytype":"detail","filename":"Extra_tyrosine_carbohydrate-binding_module.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensefree":"This is an Accepted Manuscript of an article published by Taylor & Francis in 'Biochemistry & Molecular Biology' on 03 Jan 2015, available online: http://www.tandfonline.com/10.1080/09168451.2014.996203 .","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Extra_tyrosine_carbohydrate-binding_module.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/17843/files/Extra_tyrosine_carbohydrate-binding_module.pdf"},"version_id":"350aefb1-bd2d-449e-bec3-19e265d2405b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"carbohydrate-binding module family 1","subitem_subject_scheme":"Other"},{"subitem_subject":"Irpex lacteus","subitem_subject_scheme":"Other"},{"subitem_subject":"glycoside hydrolase family 10 xylanase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1222"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-02-03"},"publish_date":"2016-02-03","publish_status":"0","recid":"17843","relation_version_is_last":true,"title":["Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:12:28.216761+00:00"}