{"created":"2021-03-01T06:20:57.882441+00:00","id":17912,"links":{},"metadata":{"_buckets":{"deposit":"8f3d96af-e870-44d0-944c-4efb5faa981d"},"_deposit":{"id":"17912","owners":[],"pid":{"revision_id":0,"type":"depid","value":"17912"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00017912","sets":["1309:1310"]},"author_link":["50012","50013","50014"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"82","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"MOLECULAR BREEDING"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We report recombinase-mediated cassette exchange (RMCE), which can permit integration of transgenes into pre-defined chromosomal loci with no co-expressed marker gene by using Agrobacterium-mediated transformation. Transgenic tobacco plants which have a single copy of negative marker genes (codA) at target loci in heterozygous and homozygous conditions were used for gene exchange by the RMCE method. By negative selection, we were able to obtain five heterozygous and four homozygous transgenic plants in which the genes were exchanged from 64 leaf segments of heterozygous and homozygous target plants, respectively. Except for one transgenic plant with an extra copy, the other eight plants had only a single copy of marker-free transgenes, and no footprint of random integrated copies was detected in half of the eight plants. The RMCE re-transformation frequencies were calculated as 6.25 % per explant and were approximately the same as the average percentage of intact single-copy transformation events for standard tobacco Agrobacterium-mediated transformation.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"MOLECULAR BREEDING. 35(2):82 (2015)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Ebinuma, Hiroyasu","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.gechjFkV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000349765300026"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SPRINGER"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1007/s11032-015-0215-7"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s11032-015-0215-7","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2015 Open Access  This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1380-3743","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1104032X","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ebinuma,  Hiroyasu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakahama,  Katsuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nanto,  Kazuya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-03-23"}],"displaytype":"detail","filename":"Enrichments_of_gene_replacement_events.pdf","filesize":[{"value":"431.1 kB"}],"format":"application/pdf","licensefree":"© The Author(s) 2015 Open Access  This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Enrichments_of_gene_replacement_events.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/17912/files/Enrichments_of_gene_replacement_events.pdf"},"version_id":"ac2d38a9-3854-4bb6-bd4d-416eb49e0019"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RMCE","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene replacement","subitem_subject_scheme":"Other"},{"subitem_subject":"Negative selection","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-03-23"},"publish_date":"2016-03-23","publish_status":"0","recid":"17912","relation_version_is_last":true,"title":["Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:12:36.519967+00:00"}