{"created":"2021-03-01T06:21:04.378194+00:00","id":18017,"links":{},"metadata":{"_buckets":{"deposit":"fa29a700-f826-4dbb-b939-54c158d35521"},"_deposit":{"id":"18017","owners":[],"pid":{"revision_id":0,"type":"depid","value":"18017"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00018017","sets":["1016:1017"]},"author_link":["50381","50382","50383","50384","50385","50386","50387","50388","50389","50390"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-11-15","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"MICROBIAL CELL FACTORIES"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. Methods: We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-HO were evaluated in mice with dextran sulfate sodium (DSS)-induced acute colitis as a model of inflammatory bowel diseases (IBD). Results: We identified the secretion of rmHO-1 by NZ-HO. rmHO-1 was biologically active as determined with spectroscopy. Viable NZ-HO was directly delivered to the colon via oral administration, and rmHO-1 was secreted onto the colonic mucosa in mice. Acute colitis in mice was induced by free drinking of 3 % DSS in water and was accompanied by an increase in the disease activity index score and histopathological changes. Daily oral administration of NZ-HO significantly improved these colitis-associated symptoms. In addition, NZ-HO significantly increased production of the anti-inflammatory cytokine interleukin (IL)-10 and decreased the expression of pro-inflammatory cytokines such as IL-1 alpha and IL-6 in the colon compared to a vector control strain. Conclusions: Oral administration of NZ-HO alleviates DSS-induced acute colitis in mice. Our results suggest that NZ-HO may be a useful mucosal therapeutic agent for treating IBD.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"MICROBIAL CELL FACTORIES. 14:189 (2015)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Ihara, Masaki","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html"},{"subitem_link_text":"Shimosato, Takeshi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000365270300002"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BIOMED CENTRAL LTD"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"26608030"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/26608030/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1186/s12934-015-0378-2"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/s12934-015-0378-2","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © Shigemori et al. 2015"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1475-2859","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12039472","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shigemori,  Suguru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe,  Takafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kudoh,  Kai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ihara,  Masaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nigar,  Shireen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto,  Yoshinari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suda,  Yoshihito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato,  Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitazawa,  Haruki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosato,  Takeshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-21"}],"displaytype":"detail","filename":"Oral_delivery_Lactococcus_lactis_secretes.pdf","filesize":[{"value":"6.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Oral_delivery_Lactococcus_lactis_secretes.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/18017/files/Oral_delivery_Lactococcus_lactis_secretes.pdf"},"version_id":"6d46e95b-7d8d-4614-ab62-435afb139266"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Lactococcus lactis","subitem_subject_scheme":"Other"},{"subitem_subject":"Heme oxygenase-1","subitem_subject_scheme":"Other"},{"subitem_subject":"Acute colitis","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-10","subitem_subject_scheme":"Other"},{"subitem_subject":"Mice","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammatory bowel disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-22"},"publish_date":"2016-04-22","publish_status":"0","recid":"18017","relation_version_is_last":true,"title":["Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:12:52.017740+00:00"}