@article{oai:soar-ir.repo.nii.ac.jp:00018207,
 author = {Suenaga,  Shin and Nikaido,  Nozomi and Totani,  Kazuhide and Kawasaki,  Kazunori and Ito,  Yoshihito and Yamashita,  Kazuhiko and Osada,  Mitsumasa},
 journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
 month = {Oct},
 note = {Published online 20 June 2016, The relationship between purification methods of β-chitin from squid pen and the physicochemical properties of β-chitin nanofibers (NFs) were investigated. Two types of β-chitin were prepared, with β-chitin (a → b) subjected to acid treatment for decalcification and then base treatment for deproteinization, while β-chitin (b → a) was treated in the opposite order. These β-chitins were disintegrated into NFs using wet pulverization. The β-chitin (b → a) NF dispersion has higher transmittance and viscosity than the β-chitin (a → b) NF dispersion. For the first time, we succeeded in obtaining 3D images of the β-chitin NF dispersion in water by using quick-freeze deep-etch replication with high-angle annular dark field scanning transmission electron microscopy. The β-chitin (b → a) NF dispersion has a denser and more uniform 3D network structure than the β-chitin (a → b) NF dispersion. Widths of the β-chitin (a → b) and (b → a) NFs were approximately 8–25 and 3–10 nm, respectively., Article, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 91:987-993 (2016)},
 pages = {987--993},
 title = {Effect of purification method of β-chitin from squid pen on the properties of β-chitin nanofibers},
 volume = {91},
 year = {2016}
}