{"created":"2021-03-01T06:21:31.950211+00:00","id":18472,"links":{},"metadata":{"_buckets":{"deposit":"8cf37f8e-15a4-4de8-a08b-1e78b32d23cc"},"_deposit":{"id":"18472","owners":[],"pid":{"revision_id":0,"type":"depid","value":"18472"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00018472","sets":["1309:1310"]},"author_link":["51461","51462","51463","51464","51465"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"522","bibliographicPageStart":"517","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chitiniphilus shinanonensis strain SAY3(T) is a chitinolytic bacterium isolated from moat water of Ueda Castle in Nagano Prefecture, Japan. Fifteen genes encoding putative chitinolytic enzymes (chiA-chiO) have been isolated from this bacterium. Five of these constitute a single operon (chiCDEFG). The open reading frames of chiC, chiD, chiE, and chiG show sequence similarity to family 18 chitinases, while chiF encodes a polypeptide with two chitin-binding domains but no catalytic domain. Each of the five genes was successfully expressed in Escherichia coli, and the resulting recombinant proteins were characterized. Four of the recombinant proteins (ChiC, ChiD, ChiE, and ChiG) exhibited endo-type chitinase activity toward chitinous substrates, while ChiF showed no chitinolytic activity. In contrast to most endo-type chitinases, which mainly produce a dimer of N-acetyl-D-glucosamine (GlcNAc) as final product, ChiG completely split the GlcNAc dimer into GlcNAc monomers, indicating that it is a novel chitinase.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. 76(3):517-522 (2012)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Nogawa, Masahiro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.gNcajNnU.html"},{"subitem_link_text":"Taguchi, Goro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"},{"subitem_link_text":"Shimosaka, Makoto","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000302667600014"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"TAYLOR & FRANCIS LTD"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"22451394"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=22451394","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1271/bbb.110822"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1271/bbb.110822","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2014, available online: http://www.tandfonline.com/ , https://doi.org/10.1271/bbb.110822 ."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Huang,  Lanxiang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shizume,  Arisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nogawa,  Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taguchi,  Goro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosaka,  Makoto"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"Heterologous_Expression_Functional_Characterization_of_Novel_Chitinase.pdf","filesize":[{"value":"752.0 kB"}],"format":"application/pdf","licensefree":"This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2014, available online: http://www.tandfonline.com/ , https://doi.org/10.1271/bbb.110822 .","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Heterologous_Expression_Functional_Characterization_of_Novel_Chitinase.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/18472/files/Heterologous_Expression_Functional_Characterization_of_Novel_Chitinase.pdf"},"version_id":"539acffc-b54f-41b3-ad17-dc494d13c981"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chitiniphilus shinanonensis","subitem_subject_scheme":"Other"},{"subitem_subject":"chitinase","subitem_subject_scheme":"Other"},{"subitem_subject":"chitin-binding domain","subitem_subject_scheme":"Other"},{"subitem_subject":"N-acetyl-D-glucosamine","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-10-28"},"publish_date":"2016-10-28","publish_status":"0","recid":"18472","relation_version_is_last":true,"title":["Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:13:45.269460+00:00"}