{"created":"2021-03-01T06:21:38.989304+00:00","id":18586,"links":{},"metadata":{"_buckets":{"deposit":"c65f033a-b4ef-4cf3-b574-2ec1a3623102"},"_deposit":{"id":"18586","owners":[],"pid":{"revision_id":0,"type":"depid","value":"18586"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00018586","sets":["1016:1017"]},"author_link":["102847","102848","102849","102850","102851","102852","102853"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"349","bibliographicPageStart":"341","bibliographicVolumeNumber":"101","bibliographic_titles":[{"bibliographic_title":"APPLIED MICROBIOLOGY AND BIOTECHNOLOGY"}]}]},"item_6_description_19":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Epub 2016 Oct 8.","subitem_description_type":"Other"}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Ihara, Masaki","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html"},{"subitem_link_text":"Ogita, Tasuku","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html"},{"subitem_link_text":"Shimosato, Takeshi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SPRINGER"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"27722916"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/27722916/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1007/s00253-016-7907-8"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00253-016-7907-8","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The original publication is available at www.springerlink.com"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0175-7598","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10503386","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shigemori,  Suguru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ihara,  Masaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato,  Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto,  Yoshinari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nigar,  Shireen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogita,  Tasuku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosato,  Takeshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-08"}],"displaytype":"detail","filename":"Secretion_immunoreactive_single-chain_variable_fragment.pdf","filesize":[{"value":"4.8 MB"}],"format":"application/pdf","licensefree":"The original publication is available at www.springerlink.com","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Secretion_immunoreactive_single-chain_variable_fragment.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/18586/files/Secretion_immunoreactive_single-chain_variable_fragment.pdf"},"version_id":"bf94d0b3-17e1-458d-b76a-4462eefd5bda"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Immunoreactivity","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin 6","subitem_subject_scheme":"Other"},{"subitem_subject":"Lactococcus lactis","subitem_subject_scheme":"Other"},{"subitem_subject":"gmLAB","subitem_subject_scheme":"Other"},{"subitem_subject":"scFv antibody","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-01"},"publish_date":"2017-02-01","publish_status":"0","recid":"18586","relation_version_is_last":true,"title":["Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:39:00.325436+00:00"}