{"created":"2021-03-01T06:22:01.596647+00:00","id":18952,"links":{},"metadata":{"_buckets":{"deposit":"029e3ae9-6bad-45af-99e9-974588bf993e"},"_deposit":{"id":"18952","owners":[],"pid":{"revision_id":0,"type":"depid","value":"18952"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00018952","sets":["1309:1310"]},"author_link":["103680","103681","103682","103683","103684","103685"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"448","bibliographicPageStart":"437","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"PLANT JOURNAL"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"C-Glycosides are characterized by their C-C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C-Glycosides are remarkably stable, as their C-C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C-glycosylflavonoids","subitem_description_type":"Abstract"},{"subitem_description":"however, the genes encoding for enzymes that catalyze C-glycosylation of flavonoids have been identified only from Oryza sativa (rice) and Zea mays (maize), and have not been identified from dicot plants. In this study, we identified the C-glucosyltransferase gene from the dicot plant Fagopyrum esculentumM. (buckwheat). We purified two isozymes from buckwheat seedlings that catalyze C-glucosylation of 2-hydroxyflavanones, which are expressed specifically in the cotyledon during seed germination. Following purification we isolated the cDNA corresponding to each isozyme [FeCGTa (UGT708C1) and FeCGTb (UGT708C2)]. When expressed in Escherichia coli, both proteins demonstrated C-glucosylation activity towards 2-hydroxyflavanones, dihydrochalcone, trihydroxyacetophenones and other related compounds with chemical structures similar to 2,4,6-trihydroxyacetophenone. Molecular phylogenetic analysis of plant glycosyltransferases shows that flavonoid C-glycosyltransferases form a different clade with other functionally analyzed plant glycosyltransferases.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLANT JOURNAL. 80(3):437-448 (2014)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Shimosaka, Makoto","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"},{"subitem_link_text":"Taguchi, Goro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000343877900005"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"WILEY-BLACKWELL"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"25142187"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25142187","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1111/tpj.12645"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/tpj.12645","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is the peer reviewed version of the following article: Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M. and Taguchi, G. (2014), Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon. Plant J, 80: 437–448., which has been published in final form at https://doi.org/10.1111/tpj.12645 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0960-7412","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10846815","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nagatomo, Yoshihisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Usui, Shiori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Takamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosaka, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taguchi, Goro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-23"}],"displaytype":"detail","filename":"Purification_molecular_cloning_functional_characterization.pdf","filesize":[{"value":"9.7 MB"}],"format":"application/pdf","licensefree":"This is the peer reviewed version of the following article: Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M. and Taguchi, G. (2014), Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon. Plant J, 80: 437–448., which has been published in final form at https://doi.org/10.1111/tpj.12645 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Purification_molecular_cloning_functional_characterization.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/18952/files/Purification_molecular_cloning_functional_characterization.pdf"},"version_id":"559d451e-ea03-45b5-ae04-23a00cc8bee2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"flavonoid C-glucosyltransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"buckwheat","subitem_subject_scheme":"Other"},{"subitem_subject":"Fagopyrum esculentum","subitem_subject_scheme":"Other"},{"subitem_subject":"cotyledon","subitem_subject_scheme":"Other"},{"subitem_subject":"2-hydroxyflavanone","subitem_subject_scheme":"Other"},{"subitem_subject":"germination","subitem_subject_scheme":"Other"},{"subitem_subject":"vitexin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-05-23"},"publish_date":"2017-05-23","publish_status":"0","recid":"18952","relation_version_is_last":true,"title":["Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:21:52.803351+00:00"}