{"_buckets": {"deposit": "7303b979-d969-42fd-835f-f3e1a5f6dcf3"}, "_deposit": {"id": "18956", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18956"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018956", "sets": []}, "author_link": ["103690"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-03-31", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2016-03-31"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。FREE RADICAL RESEARCH. 50(4):414-425 (2016); doi:10.3109/10715762.2015.1134795.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "山田　靖. Lipocalin 2 attenuates iron-related oxidative stress and prolongs the survival of ovarian clear cell carcinoma cells by up-regulating the CD44 variant（リポカリン2は変異型CD44の発現亢進を介して卵巣明細胞癌細胞の鉄関連酸化ストレスを減弱し、生存を促進する）. 信州大学, 2016, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601甲第1059号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000371933700005"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "26729415"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/26729415/", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.3109/10715762.2015.1134795"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.3109/10715762.2015.1134795", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is an Accepted Manuscript of an article published by Taylor \u0026 Francis in Free Radical Research on 12/02/2016, available online: http://www.tandfonline.com/10.3109/10715762.2015.1134795"}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "医学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "甲第1059号"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Cystine transporter; lipocalin 2; ovarian clear cell carcinoma; ovarian endometriosis; oxidative stress; reactive oxygen species (ROS)"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "GELATINASE-ASSOCIATED LIPOCALIN; GASTRIC-CANCER; CARCINOGENESIS; EXPRESSION; NGAL; GLUTATHIONE; RECEPTOR; DISEASE; TRANSPORTERS; PROGRESSION"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Ovarian clear cell carcinoma (CCC) arises from ovarian endometriosis. Intra-cystic fluid contains abundant amounts of free iron, which causes persistent oxidative stress, a factor that has been suggested to induce malignant transformation. However, the mechanisms linking oxidative stress and carcinogenesis in CCC currently remain unclear. Lipocalin 2 (LCN2), a multifunctional secretory protein, functions as an iron transporter as well as an antioxidant. Therefore, we herein examined the roles of LCN2 in the regulation of intracellular iron concentrations, oxidative stress, DNA damage, and antioxidative functions using LCN2-overexpressing (ES2), and LCN2-silenced (RMG-1) CCC cell lines. The results of calcein staining indicated that the up-regulated expression of LCN2 correlated with increases in intracellular iron concentrations. However, a DCFH-DA assay and 8OHdG staining revealed that LCN2 reduced intracellular levels of reactive oxygen species and DNA damage. Furthermore, the expression of LCN2 suppressed hydrogen peroxide-induced apoptosis and prolonged cell survival, suggesting an antioxidative role for LCN2. The expression of mRNAs and proteins for various oxidative stress-catalyzing enzymes, such as heme oxygenase (HO), superoxide dismutase (SOD), and glutathione peroxidase, was not affected by LCN2, whereas the intracellular concentration of the potent antioxidant, glutathione (GSH), was increased by LCN2. Furthermore, the expression of xCT, a cystine transporter protein, and CD44 variant 8-10 (CD44v), a stem cell marker, was up-regulated by LCN2. Although LCN2 increased intracellular iron concentrations, LCN2-induced GSH may catalyze and override oxidative stress via CD44v and xCT, and subsequently enhance the survival of CCC cells in oxidative stress-rich endometriosis."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "山田, 靖", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "103690", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "12MH043B_shinsa.pdf", "filesize": [{"value": "93.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 93100.0, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/18956/files/12MH043B_shinsa.pdf"}, "version_id": "ef90cb40-2670-4ea9-aa3b-70486ffa7722"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "12MH043B_yoshi.pdf", "filesize": [{"value": "129.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 129000.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/18956/files/12MH043B_yoshi.pdf"}, "version_id": "34a936e0-8a5d-436c-bad3-21577ab76b8c"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-07-10"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "12MH043B_ronbun.pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This is an Accepted Manuscript of an article published by Taylor \u0026 Francis in Free Radical Research on 12/02/2016, available online: http://www.tandfonline.com/10.3109/10715762.2015.1134795", "licensetype": "license_note", "mimetype": "application/pdf", "size": 2700000.0, "url": {"label": "博士論文の全文", "objectType": "fulltext", "url": "https://soar-ir.repo.nii.ac.jp/record/18956/files/12MH043B_ronbun.pdf"}, "version_id": "381aabc3-3783-4f53-b4a0-b372fddf4c5c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Lipocalin 2 attenuates iron-related oxidative stress and prolongs the survival of ovarian clear cell carcinoma cells by up-regulating the CD44 variant（リポカリン2は変異型CD44の発現亢進を介して卵巣明細胞癌細胞の鉄関連酸化ストレスを減弱し、生存を促進する）", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Lipocalin 2 attenuates iron-related oxidative stress and prolongs the survival of ovarian clear cell carcinoma cells by up-regulating the CD44 variant（リポカリン2は変異型CD44の発現亢進を介して卵巣明細胞癌細胞の鉄関連酸化ストレスを減弱し、生存を促進する）", "subitem_title_language": "en"}]}, "item_type_id": "14", "owner": "1", "path": ["849"], "permalink_uri": "http://hdl.handle.net/10091/00019718", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-06-06"}, "publish_date": "2017-06-06", "publish_status": "0", "recid": "18956", "relation": {}, "relation_version_is_last": true, "title": ["Lipocalin 2 attenuates iron-related oxidative stress and prolongs the survival of ovarian clear cell carcinoma cells by up-regulating the CD44 variant（リポカリン2は変異型CD44の発現亢進を介して卵巣明細胞癌細胞の鉄関連酸化ストレスを減弱し、生存を促進する）"], "weko_shared_id": -1}