@article{oai:soar-ir.repo.nii.ac.jp:00019363,
 author = {Nomura, Takaomi and Ito, Miho and Kanamori, Mai and Shigeno, Yuta and Uchiumi, Toshio and Arai, Ryoichi and Tsukada, Masuhiro and Hirabayashi, Kimio and Ohkawa, Kousaku},
 issue = {2},
 journal = {Biochemical and Biophysical Research Communications},
 month = {Jan},
 note = {Larval Stenopsyche marmorata constructs food capture nets and fixed retreats underwater using self-produced proteinaceous silk fibers. In the Chikuma River (Nagano Prefecture, Japan) S. marmorata has a bivoltine life cycle; overwintering larvae grow slowly with reduced net spinning activity in winter. We recently reported constant transcript abundance of S. marmorata silk protein 1 (Smsp-1), a core S. marmorata silk fiber component, in all seasons, implying translational suppression in the silk gland during winter. Herein, we prepared and characterized silk gland ribosomes from seasonally collected S. marmorata larvae. Ribosomes from silk glands immediately frozen in liquid nitrogen (LN2) after dissection exhibited comparable translation elongation activity in spring, summer, and autumn. Conversely, silk glands obtained in winter did not contain active ribosomes and Smsp-1. Ribosomes from silk glands immersed in ice-cold physiological saline solution for approximately 4 h were translationally inactive, despite summer collection and Smsp-1 expression. The ribosomal inactivation occurs because of defects in the formation of 80S ribosomes, presumably due to splitting of 60S subunits containing 28S rRNA with central hidden break, in response to cold stress. These results suggest a novel-type ribosome-regulated translation control mechanism., Article, Biochemical and Biophysical Research Communications.469(2):210-215(2016)},
 pages = {210--215},
 title = {Characterization of silk gland ribosomes from a bivoltine caddisfly, Stenopsyche marmorata: translational suppression of a silk protein in cold conditions},
 volume = {469},
 year = {2016}
}