{"created":"2021-07-28T01:31:19.437651+00:00","id":2000345,"links":{},"metadata":{"_buckets":{"deposit":"dad5a12d-13f1-4bad-b0c9-b3cbc8c9f5bd"},"_deposit":{"created_by":123,"id":"2000345","owner":"123","owners":[123],"pid":{"revision_id":0,"type":"depid","value":"2000345"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02000345","sets":["461:462"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"14","bibliographicPageStart":"7752","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration, osteoconductive, and osteoinductive characteristics of biomaterials. However, the evaluation of biomaterial osteogenesis-inducing capacity using osteoblast-like cells is not standardized; instead, it is performed under laboratory-specific culture conditions with different culture media. However, the effect of different media conditions on bone formation has not been investigated. Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions were tested in uncoated plates and plates coated with collagen type I and poly-L-lysine, highly biocompatible molecules commonly used as pseudobiomaterials. We found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may affect biomaterial regeneration. We posit that an in vitro model that recapitulates in vivo bone formation should be established before evaluating biomaterials.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences. 22(14):7752 (2021)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Sobajima Atsushi","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.uULePpym.html"},{"subitem_link_text":"Uemura Takeshi","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.HmANWayV.html"},{"subitem_link_text":"Saito Naoto","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.WpkhuUkh.html"},{"subitem_link_text":"Haniu, Hisao","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"34299372"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/34299372/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3390/ijms22147752"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms22147752","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"EISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Izumiya, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Haniu, Miyu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ueda, Katsuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishida, Haruka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ma, Chuang","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ideta, Hirokazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sobajima, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ueshiba, Koki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Uemura, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saito, Naoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Haniu, Hisao","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-07-28"}],"displaytype":"detail","filename":"ijms-22-07752-v3.pdf","filesize":[{"value":"4.7 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"ijms-22-07752-v3.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/2000345/files/ijms-22-07752-v3.pdf"},"version_id":"892e8674-4703-4e58-985a-6edb8a5c39bf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"biomaterials","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vitamin C","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"calcification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell culture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"collagen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"alkaline phosphatase","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media","subitem_title_language":"en"}]},"item_type_id":"6","owner":"123","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-07-28"},"publish_date":"2021-07-28","publish_status":"0","recid":"2000345","relation_version_is_last":true,"title":["Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media"],"weko_creator_id":"123","weko_shared_id":-1},"updated":"2022-12-14T04:41:17.936450+00:00"}