{"created":"2021-09-28T23:55:55.890022+00:00","id":2000388,"links":{},"metadata":{"_buckets":{"deposit":"b3d704f8-93d0-436b-8268-d2d23956ecdd"},"_deposit":{"created_by":16,"id":"2000388","owner":"16","owners":[16],"owners_ext":{"displayname":"shinshu_univ","username":"shinshu_univ"},"pid":{"revision_id":0,"type":"depid","value":"2000388"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02000388","sets":["1309:1310"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1802","bibliographicPageStart":"1790","bibliographicVolumeNumber":"82","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Rutin, a 3-rutinosyl quercetin, is a representative flavonoid distributed in many plant species, and is highlighted for its therapeutic potential. In this study, we purified uridine diphosphate-rhamnose: quercetin 3-O-glucoside 6″-O-rhamnosyltransferase and isolated the corresponding cDNA (FeF3G6″RhaT) from seedlings of common buckwheat (Fagopyrum esculentum). The recombinant FeF3G6″RhaT enzyme expressed in Escherichia coli exhibited 6″-O-rhamnosylation activity against flavonol 3-O-glucoside and flavonol 3-O-galactoside as substrates, but showed only faint activity against flavonoid 7-O-glucosides. Tobacco cells expressing FeF3G6″RhaT converted the administered quercetin into rutin, suggesting that FeF3G6″RhaT can function as a rhamnosyltransferase in planta. Quantitative PCR analysis on several organs of common buckwheat revealed that accumulation of FeF3G6″RhaT began during the early developmental stages of rutin-accumulating organs, such as flowers, leaves, and cotyledons. These results suggest that FeF3G6″RhaT is involved in rutin biosynthesis in common buckwheat.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Bioscience, Biotechnology, and Biochemistry. 82(10):1790–1802(2018)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"下坂, 誠","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"},{"subitem_link_text":"田口, 悟朗","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/29972345","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1080/09168451.2018.1491286","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-6947","subitem_source_identifier_type":"EISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12018610","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koja, Eiki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohata, Soichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Maruyama, Yoshinori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Hideyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimosaka, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Taguchi, Goro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-09-28"}],"filename":"1491286.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"url":"https://soar-ir.repo.nii.ac.jp/record/2000388/files/1491286.pdf"},"version_id":"eb7a775f-cc2e-46d9-a7fa-cc63cb726c3f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"common buckwheat","subitem_subject_scheme":"Other"},{"subitem_subject":"flavonoid biosynthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"rhamnosyltransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"rutin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)","subitem_title_language":"en"}]},"item_type_id":"6","owner":"16","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-09-29"},"publish_date":"2021-09-29","publish_status":"0","recid":"2000388","relation_version_is_last":true,"title":["Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2022-12-14T04:28:40.111417+00:00"}