{"created":"2022-01-27T05:02:25.906054+00:00","id":2000704,"links":{},"metadata":{"_buckets":{"deposit":"60ba368a-c6ac-48e0-8c62-4613e93794e0"},"_deposit":{"id":"2000704","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000704"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02000704","sets":["1016:1017"]},"author_link":[],"control_number":"2000704","item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-04-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"160","bibliographicPageStart":"151","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Journal of Nutritional Science and Vitaminology"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract\nCacao extract (CE) consumption has beneficial effects on human health, such as lowering the risk of obesity. However, the underlying molecular mechanism for the anti-obesity effect of CE remains incompletely understood. Here, we used a 50% aqueous alcohol extract of cacao mass, which is rich in methylxanthine derivatives (about 11%) and poor in flavan-3-ols (less than 1%), and assessed the suppression effects of this extract on adipocyte differentiation to investigate the anti-obesity mechanism. CE dose-dependently decreased fat accumulation in 3T3-L1 cells without affecting cell viability. CE also dose-dependently decreased the protein and gene expression levels of two adipogenesis-related transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs). Moreover, CE decreased protein expression levels of sterol regulatory element-binding protein 1 (SREBP1) and its downstream fatty acid synthase (FAS), which was accompanied by the retained localization of SREBP1 in the cytoplasm of 3T3-L1 cells. After ICR mice were fed a diet containing 1% CE for 1 wk, their white adipose tissue weight was lower, whereas their brown adipose tissue weight was higher compared with those of control animals. Additionally, the protein expression levels of PPARγ, C/EBPs, SREBP1, and FAS in the white adipose tissue of these mice were also lower than those in control animals. In contrast, diet supplementation with CE induced higher levels of phosphorylated AMP-activated protein kinase (AMPK) and its downstream acetyl-CoA carboxylase. In conclusion, methylxanthine derivative-rich CE decreases fat accumulation in adipocytes by downregulating the expression of the adipocyte differentiation master regulators through the activation of AMPK.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Nutritional Science and Vitaminology. 64(2): 151-160. (2018)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"三谷, 塁一","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"一般財団法人 学会誌刊行センター"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3177/jnsv.64.151"}],"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3177/jnsv.64.151","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-7742","subitem_source_identifier_type":"EISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12108939","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamashita, Yoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mitani, Takakazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Wang, Liuqing","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ashida, Hitoshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-27"}],"filename":"JNSV64-2-151.pdf","format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JNSV64-2-151.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/2000704/files/JNSV64-2-151.pdf"},"version_id":"b4a7052d-b246-4939-b89b-192aa90a3a30"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cacao","subitem_subject_scheme":"Other"},{"subitem_subject":"methylxanthine derivatives","subitem_subject_scheme":"Other"},{"subitem_subject":"PPARγ","subitem_subject_scheme":"Other"},{"subitem_subject":"C/EBPs","subitem_subject_scheme":"Other"},{"subitem_subject":"adipocyte differentiation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-01-27"},"publish_date":"2022-01-27","publish_status":"0","recid":"2000704","relation_version_is_last":true,"title":["Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:19:36.026742+00:00"}