{"created":"2022-09-12T01:52:22.731829+00:00","id":2001061,"links":{},"metadata":{"_buckets":{"deposit":"cc09578b-d94e-4641-8e10-400111f870f3"},"_deposit":{"id":"2001061","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001061"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02001061","sets":["1016:1017"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"120","bibliographicPageStart":"111","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"Muscles","bibliographic_titleLang":"en"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cancer cachexia is characterized by irreversible muscle loss which is a critical factor in the prognosis of cancer patients. Myoblasts are myogenic precursor cells that are required to maintain skeletal muscle tissue. Previous studies reported that cancer-released factors deteriorate myoblast differentiation, which is one of the causes of cachexia-associated muscle wasting. We recently identified the myogenetic oligodeoxynucleotide, iSN04, which serves as an anti-nucleolin aptamer and promotes myogenesis. The present study investigated the effects of iSN04 on human myoblasts exposed to a conditioned medium (CM) of cancer cells. CM of colon cancer cell lines LoVo and HCT-116 significantly impaired myogenic differentiation and the myotube formation of human myoblasts by inducing the expression of inflammatory cytokines such as interleukin (IL)-1β and tumor necrosis factor-α (TNF-α); however, the CM of the colon fibroblast cell line CCD-18Co did not. Intriguingly, iSN04 completely reversed the deterioration of myoblast differentiation by LoVo-CM by upregulating MyoD and myogenin, and downregulating myostatin, IL-1β, and TNF-α. TNF-α, of which a high level was produced in LoVo, alone inhibited myogenic differentiation and induced IL-1β, IL-6, and IL-8 transcriptions of myoblasts; however, pre-treatment with iSN04 reversed TNF-α-induced cachectic phenotypic features. The results indicate that iSN04 protects myoblasts against the effects of cancer-released factors and maintains their myogenic activity. This study provides a novel therapeutic strategy to prevent muscle loss associated with cancer cachexia.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Muscles. 1(2) : 111-120 (2022)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"下里, 剛士","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"},{"subitem_link_text":"高谷, 智英","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3390/muscles1020012"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/muscles1020012","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights_resource":"Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2813-0413","subitem_source_identifier_type":"EISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nihashi, Yuma","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamamoto, Machi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimosato, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takaya, Tomohide","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-13"}],"filename":"Muscles1-111.pdf","format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://soar-ir.repo.nii.ac.jp/record/2001061/files/Muscles1-111.pdf"},"version_id":"7bff8c3a-c4cf-465f-a6d3-6ce7daf71bae"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"aptamer","subitem_subject_scheme":"Other"},{"subitem_subject":"cachexia","subitem_subject_scheme":"Other"},{"subitem_subject":"inflammatory response","subitem_subject_scheme":"Other"},{"subitem_subject":"myoblast","subitem_subject_scheme":"Other"},{"subitem_subject":"myogenetic oligodeoxynucleotide","subitem_subject_scheme":"Other"},{"subitem_subject":"myogenic differentiation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Myogenetic Oligodeoxynucleotide Restores Differentiation and Reverses Inflammation of Myoblasts Aggravated by Cancer-Conditioned Medium","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Myogenetic Oligodeoxynucleotide Restores Differentiation and Reverses Inflammation of Myoblasts Aggravated by Cancer-Conditioned Medium","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-09-13"},"publish_date":"2022-09-13","publish_status":"0","recid":"2001061","relation_version_is_last":true,"title":["Myogenetic Oligodeoxynucleotide Restores Differentiation and Reverses Inflammation of Myoblasts Aggravated by Cancer-Conditioned Medium"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:22:59.477455+00:00"}