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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells

http://hdl.handle.net/10091/0002001217
http://hdl.handle.net/10091/0002001217
25ce1e38-e677-4860-9dee-0d940ce45cee
名前 / ファイル ライセンス アクション
Biomedicines10_2691.pdf Biomedicines10_2691.pdf
Item type 学術雑誌論文 / Journal Article(1)
公開日 2022-10-26
タイトル
言語 en
タイトル Myogenetic Oligodeoxynucleotides as Anti-Nucleolin Aptamers Inhibit the Growth of Embryonal Rhabdomyosarcoma Cells
言語
言語 eng
キーワード
主題Scheme Other
主題 aptamer
キーワード
主題Scheme Other
主題 embryonal
キーワード
主題Scheme Other
主題 rhabdomyosaycoma
キーワード
主題Scheme Other
主題 myogenetic oligodeoxynucleotide
キーワード
主題Scheme Other
主題 nucleolin
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Nohira, Naoki

× Nohira, Naoki

en Nohira, Naoki

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Shinji, Sayaka

× Shinji, Sayaka

en Shinji, Sayaka

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Nakamura, Shunichi

× Nakamura, Shunichi

en Nakamura, Shunichi

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Nihashi, Yuma

× Nihashi, Yuma

en Nihashi, Yuma

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Shimosato, Takeshi

× Shimosato, Takeshi

en Shimosato, Takeshi

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Takaya, Tomohide

× Takaya, Tomohide

en Takaya, Tomohide

Search repository
信州大学研究者総覧へのリンク
氏名 下里, 剛士
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html
信州大学研究者総覧へのリンク
氏名 高谷, 智英
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html
出版者
出版者 MDPI
引用
内容記述タイプ Other
内容記述 Biomedicines 10(11) : 2691-(2022)
書誌情報 en : Biomedicines

巻 10, 号 11, p. 2691, 発行日 2022-10-25
抄録
内容記述タイプ Abstract
内容記述 Embryonal rhabdomyosarcoma (ERMS) is the muscle-derived tumor retaining myogenic ability. iSN04 and AS1411, which are myogenetic oligodeoxynucleotides (myoDNs) serving as anti-nucleolin aptamers, have been reported to inhibit the proliferation and induce the differentiation of myoblasts. The present study investigated the effects of iSN04 and AS1411 in vitro on the growth of multiple patient-derived ERMS cell lines, ERMS1, KYM1, and RD. RT-PCR and immunostaining revealed that nucleolin was abundantly expressed and localized in nucleoplasm and nucleoli in all ERMS cell lines, similar to myoblasts. Both iSN04 and AS1411 at final concentrations of 10–30 μM significantly decreased the number of all ERMS cells; however, their optimal conditions were different among the cell lines. In all ERMS cell lines, iSN04 at a final concentration of 10 μM markedly reduced the ratio of EdU+ cells, indicating the inhibition of cell proliferation. Quantitative RT-PCR or immunostaining of phosphorylated histone H3 and myosin heavy chain demonstrated that iSN04 suppressed the cell cycle and partially promoted myogenesis but did not induce apoptosis in ERMS cells. Finally, both iSN04 and AS1411 at final concentrations of 10–30 μM disrupted the formation and outgrowth of RD tumorspheres in three-dimensional culture mimicking in vivo tumorigenesis. In conclusion, ERMS cells expressed nucleolin, and their growth was inhibited by the anti-nucleolin aptamers, iSN04 and AS1411, which modulates several cell cycle-related and myogenic gene expression. The present study provides evidence that anti-nucleolin aptamers can be used as nucleic acid drugs for chemotherapy against ERMS.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ EISSN
収録物識別子 2227-9059
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 https://doi.org/10.3390/biomedicines10112691
関連名称 10.3390/biomedicines10112691
権利
権利情報Resource Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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