{"created":"2022-11-14T02:56:01.881861+00:00","id":2001232,"links":{},"metadata":{"_buckets":{"deposit":"14a3c09c-b87b-48c8-8de4-191b4a2c1963"},"_deposit":{"id":"2001232","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001232"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02001232","sets":["461:462"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-10-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageStart":"e01661-19","bibliographicVolumeNumber":"85","bibliographic_titles":[{"bibliographic_title":"Applied and Environmental Microbiology"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study focused on the detection of the plasmid-mediated mcr colistin resistance gene in Escherichia coli isolates from wastewater treatment plants (WWTPs). Seven influent samples were collected from three WWTPs in Nagano Prefecture, Japan, during August and December 2018. Colistin-resistant E. coli isolates were selected on colistin-supplemented CHROMagar ECC plates. mcr-1-positive isolates were subjected to whole-genome sequencing (WGS) analysis. From six influent samples, seven mcr-1-positive but extended-spectrum beta-lactamase (ESBL)-negative isolates belonging to different genetic lineages, namely, B2-O25:H4-ST131-fimH22, B2-O2:H1-ST135-fimH2, B1-O8:H9-ST764-fimH32, B1-O23:H16-5T453-fimH31, A-O81: H27-ST10-fimH54, A-O16:H5-ST871-fimH25, and F-O11:H6-ST457-fimH145, were detected. The MICs of colistin for these isolates ranged from 4 to 16 mg/liter. The mcr-1 genes were located on plasmids belonging to IncX4 and Incl2 in five and two isolates, respectively. Four IncX4 plasmids with the same size (33,309 bp) showed high sequence similarity (4 single-nucleotide variations). The remaining one IncX4 plasmid, with a size of 33,858 bp, carried the mcr-1 gene with the single synonymous nucleic substitution T27C. Two Incl2 plasmids with sizes of 60,710 bp and 60,733 bp had high sequence similarity (99.9% identity; 100% query coverage). Two of five isolates carrying IncX4 plasmids and both of the isolates carrying Incl2 plasmids harbored CoIV plasmids carrying virulence-associated genes of avian pathogenic E. coli (APEC). In addition, another isolate of the B2-O25:H4-ST131-fimH22 lineage had those APEC-associated virulence genes on its chromosome. In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterized as positive for APEC-associated virulence genes. The copresence of those genes may suggest the existence of a common source in animals and/or their associated environments.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Applied and Environmental Microbiology 85(22) : e01661-19-(2019)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"長野, 由紀子","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.OUAeumyC.html"},{"subitem_link_text":"長野, 則之","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.HULFumkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"31492666"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/31492666/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1128/AEM.01661-19"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/AEM.01661-19","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019 American Society for Microbiology. All Rights Reserved."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1098-5336","subitem_source_identifier_type":"EISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00543249","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayashi, Wataru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tanaka, Hayato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Taniguchi, Yui","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iimura, Masaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Soga, Eiji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kubo, Ryoichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsuo, Nao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawamura, Kumiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Arakawa, Yoshichika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagano, Yukiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagano, Noriyuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-14"}],"filename":"18K08428_2.pdf","format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"18K08428_2.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/2001232/files/18K08428_2.pdf"},"version_id":"b5fe4be2-def8-4f74-96bc-f4e2c83b0dbd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"WWTPs","subitem_subject_scheme":"Other"},{"subitem_subject":"mcr-1","subitem_subject_scheme":"Other"},{"subitem_subject":"APEC","subitem_subject_scheme":"Other"},{"subitem_subject":"ColV","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-11-14"},"publish_date":"2022-11-14","publish_status":"0","recid":"2001232","relation_version_is_last":true,"title":["Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:42:04.144926+00:00"}