{"created":"2022-11-14T02:56:03.729143+00:00","id":2001233,"links":{},"metadata":{"_buckets":{"deposit":"91af0e9b-a9dc-40f4-81a6-b32b16b5a664"},"_deposit":{"id":"2001233","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001233"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02001233","sets":["461:462"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-10-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageStart":"e01740-19","bibliographicVolumeNumber":"85","bibliographic_titles":[{"bibliographic_title":"Applied and Environmental Microbiology"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The presence of antimicrobial-resistant bacteria and resistance genes in aquatic environments is a serious public health concern. This study focused on Escherichia coli possessing bla(CTX-M) genes in wastewater inflows. Twelve crude inflow water samples from wastewater treatment plant (WWTP) A and two samples each from three other WWTPs were collected in 2017 and 2018. A total of 73 E. coli isolates with 31 different sequence types (STs) harboring distinctive bla(CTX-M) gene repertoires were detected. In WWTP A influents, bla(CTX-M-14) (14 isolates) was dominant, followed by bla(CTX-M-15 )(12 isolates) and bla(CTX-M-27) (10 isolates). The chimeric bla(CTX-M-)(64) and bla(CTX-M-123) genes were each identified in one of the E. coli isolates from the same WWTP A inflow port. The bla(CTX-M-27) gene was associated with five of seven B2-ST131 isolates, including three isolates of the B2-O25b-ST131-H30R/non-Rx lineage. One of the remaining two isolates belonged to the B2-O25b-ST131-H30R/Rx lineage harboring the bla(CTX-M-15) gene. As for the B2-025b-ST131-H30R/non-Rx lineage, two isolates with bla(CTX-M-27) were recovered from each of the WWTP B and D influents, and one isolate with bla(CTX-M-174) was also recovered from WWTP B influent. Whole-genome sequencing of chimeric bla(CTX-M) -harboring E. coli isolates revealed that the bla(CTX-M-64) gene was integrated into the chromosome of ST10 E. coli B22 via ISEcp1-mediated transposition of a 9,467-bp sequence. The bla(CTX-M-123)-carrying Incl1 plasmid pB64 was 109,169 bp in length with pST108. The overall findings suggest that wastewater may act as a probable reservoir of clinically significant clonal lineages mediating antimicrobial resistance genes and chimeric genes that have not yet been identified from human isolates of domestic origin in Japan.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Applied and Environmental Microbiology 85(22) : e01740-19-(2019)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"長野, 由紀子","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.OUAeumyC.html"},{"subitem_link_text":"長野, 則之","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.HULFumkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"31519655"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/31519655/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1128/AEM.01740-19"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/AEM.01740-19","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019 American Society for Microbiology. All Rights Reserved."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1098-5336","subitem_source_identifier_type":"EISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00543249","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka, Hayato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hayashi, Wataru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iimura, Masaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Taniguchi, Yui","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Soga, Eiji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsuo, Nao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawamura, Kumiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Arakawa, Yoshichika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagano, Yukiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagano, Noriyuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-14"}],"filename":"18K08428_3.pdf","format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"18K08428_3.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/2001233/files/18K08428_3.pdf"},"version_id":"39f28d08-1915-44b1-973d-7c3fa163c6ea"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"wastewater","subitem_subject_scheme":"Other"},{"subitem_subject":"ESBL","subitem_subject_scheme":"Other"},{"subitem_subject":"CTX-M-64","subitem_subject_scheme":"Other"},{"subitem_subject":"CTX-M-123","subitem_subject_scheme":"Other"},{"subitem_subject":"WWTPs","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Wastewater as a Probable Environmental Reservoir of Extended-Spectrum-β-Lactamase Genes: Detection of Chimeric β-Lactamases CTX-M-64 and CTX-M-123","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Wastewater as a Probable Environmental Reservoir of Extended-Spectrum-β-Lactamase Genes: Detection of Chimeric β-Lactamases CTX-M-64 and CTX-M-123","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-11-14"},"publish_date":"2022-11-14","publish_status":"0","recid":"2001233","relation_version_is_last":true,"title":["Wastewater as a Probable Environmental Reservoir of Extended-Spectrum-β-Lactamase Genes: Detection of Chimeric β-Lactamases CTX-M-64 and CTX-M-123"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:42:03.049278+00:00"}