@article{oai:soar-ir.repo.nii.ac.jp:02001236,
 author = {Hayashi, Wataru and Iimura, Masaki and Soga, Eiji and Koide, Shota and Izumi, Katsutoshi and Yoshida, Satoshi and Arakawa, Yoshichika and Nagano, Yukiko and Nagano, Noriyuki},
 issue = {10},
 journal = {Microbial Drug Resistance},
 month = {Oct},
 note = {The aim of this study was to investigate the presence of colistin- and/or tigecycline-resistant Klebsiella spp. in influents from four wastewater treatment plants (WWTPs), which partly reflect the gut microbiome of human populations. Colistin- and tigecycline-resistant Klebsiella pneumoniae isolates (K30/ST29) were detected four times from the WWTP A during a period of 3 months. Disruptions of the mgrB and ramR genes by ISEc68 and ISKpn21, respectively, were identified in those four isolates. They also shared the IncL/M 86,197-bp plasmids carrying a bla(CTX-M-3) and Tn1548-associated armA [IS26-IntI1-dfrA12-gucF-aadA2-qacE Delta 1-sul1-ISCR1-ISEc28-armA-ISEc29-msr(E)-mph(E)-IS26]. Those isolates formed a distinct cluster within wgMLST clusters of ST29 K30 public reference strains of human origin and were unique due to harboring of Tn21-like mercury resistance operon transposons in addition to silver, copper, and arsenic resistance determinants. Five K. pneumoniae strains with different STs and 1 Klebsiella quasipneumoniae strain, exhibiting colistin resistance, were detected in WWTPs B, C, and D. For these isolates, disruptions of mgrB by ISEc68 (three isolates) or ISEcl1 (one isolate), insertion of IS2 in the mgrB promoter region (one isolate), and inactivation of MgrB by a nonsense mutation (one isolate) were identified. Close monitoring of these mcr-negative colistin- and/or tigecycline-resistant bacteria in wastewater influents is imperative to avoid further limiting of treatment options., Article, Microbial Drug Resistance 27(10) : 1433-1442(2021)},
 pages = {1433--1442},
 title = {Presence of Colistin- and Tigecycline-Resistant Klebsiella pneumoniae ST29 in Municipal Wastewater Influents in Japan},
 volume = {27},
 year = {2021}
}