@article{oai:soar-ir.repo.nii.ac.jp:02001238,
 author = {Hayashi, Wataru and Yoshida, Satoshi and Izumi, Katsutoshi and Koide, Shota and Soga, Eiji and Takizawa, Shino and Arakawa, Yoshichika and Nagano, Yukiko and Nagano, Noriyuki},
 journal = {Journal of Global Antimicrobial Resistance},
 month = {Jun},
 note = {Objectives: We determined the whole DNA sequences of plasmids carrying a rare extended-spectrum beta-lactamase gene (bla(TEM-61)) to precisely understand the spread of resistance among nosocomial Serratia marcescens populations.  <br>Methods: Twenty non-duplicate ceftazidime-resistant S. marcescens nosocomial isolates (ceftazidime MICs, 32 to >128 mg/L) collected over 1 year were pulsotyped and nucleotide sequences of the bla(TEM-61) gene and its promoter region were determined. Twelve representative isolates were analysed by whole-genome sequencing.  <br>Results: The 20 isolates comprised two distinct pulsotypes: I (14 isolates) and II (6 isolates). They all contained the bla(TEM-61) gene. A polymorphism in the repeat number of a 15-nucleotide sequence (5'-ATGTCATGATAATAA-3') was found in the promoter region of bla(TEM-61); two, three and four repeat units were found in 6,12 and 2 isolates, respectively. Single nucleotide polymorphism (SNP)-based phylogenetic analysis of 12 isolates revealed that 7 isolates of pulsotype I (12-44 SNP differences) and 5 isolates of pulsotype II (15-55 SNP differences) formed two distinct clusters of genotypes 1 and 2, respectively. All 12 isolates harboured a plasmid carrying the Tn1-bla(TEM-61) element, although they were slightly different in size (78 883 bp, 78 898 bp and 78 913 bp) owing to differences in the number of 15-bp repetitive sequences. A 42 542-bp broad-host-range plasmid carrying the Tn1-bla(TEM-61) element was also found in one of the isolates.  Conclusions: We characterised a plasmid-encoded novel Tn1-bla(TEM-61) element and transposon-dependent mechanisms underlying the propagation of antibiotic resistance, together with repeated new polymorphic 15-bp units in the promoter of bla(TEM-61). (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy., Article, Journal of Global Antimicrobial Resistance 25 : 124-131(2021)},
 pages = {124--131},
 title = {Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare bla TEM-61},
 volume = {25},
 year = {2021}
}