{"created":"2022-11-14T02:56:07.672632+00:00","id":2001238,"links":{},"metadata":{"_buckets":{"deposit":"326007f3-906e-4aa1-b2ae-e014a1bcc42d"},"_deposit":{"id":"2001238","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001238"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02001238","sets":["461:462"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"131","bibliographicPageStart":"124","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"Journal of Global Antimicrobial Resistance"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives: We determined the whole DNA sequences of plasmids carrying a rare extended-spectrum beta-lactamase gene (bla(TEM-61)) to precisely understand the spread of resistance among nosocomial Serratia marcescens populations.  <br>Methods: Twenty non-duplicate ceftazidime-resistant S. marcescens nosocomial isolates (ceftazidime MICs, 32 to >128 mg/L) collected over 1 year were pulsotyped and nucleotide sequences of the bla(TEM-61) gene and its promoter region were determined. Twelve representative isolates were analysed by whole-genome sequencing.  <br>Results: The 20 isolates comprised two distinct pulsotypes: I (14 isolates) and II (6 isolates). They all contained the bla(TEM-61) gene. A polymorphism in the repeat number of a 15-nucleotide sequence (5'-ATGTCATGATAATAA-3') was found in the promoter region of bla(TEM-61); two, three and four repeat units were found in 6,12 and 2 isolates, respectively. Single nucleotide polymorphism (SNP)-based phylogenetic analysis of 12 isolates revealed that 7 isolates of pulsotype I (12-44 SNP differences) and 5 isolates of pulsotype II (15-55 SNP differences) formed two distinct clusters of genotypes 1 and 2, respectively. All 12 isolates harboured a plasmid carrying the Tn1-bla(TEM-61) element, although they were slightly different in size (78 883 bp, 78 898 bp and 78 913 bp) owing to differences in the number of 15-bp repetitive sequences. A 42 542-bp broad-host-range plasmid carrying the Tn1-bla(TEM-61) element was also found in one of the isolates.  Conclusions: We characterised a plasmid-encoded novel Tn1-bla(TEM-61) element and transposon-dependent mechanisms underlying the propagation of antibiotic resistance, together with repeated new polymorphic 15-bp units in the promoter of bla(TEM-61). (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Global Antimicrobial Resistance 25 : 124-131(2021)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"長野, 由紀子","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.OUAeumyC.html"},{"subitem_link_text":"長野, 則之","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.HULFumkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"33762209"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/33762209/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.jgar.2021.03.010"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jgar.2021.03.010","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2213-7173","subitem_source_identifier_type":"EISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayashi, Wataru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yoshida, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Izumi, Katsutoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koide, Shota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Soga, Eiji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takizawa, Shino","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Arakawa, Yoshichika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagano, Yukiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagano, Noriyuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-14"}],"filename":"18K08428_8.pdf","format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"18K08428_8.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/2001238/files/18K08428_8.pdf"},"version_id":"465bb6f6-46df-4665-9765-a49b85dac8a0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Serratia marcescens","subitem_subject_scheme":"Other"},{"subitem_subject":"Nosocomial spread","subitem_subject_scheme":"Other"},{"subitem_subject":"Tn1","subitem_subject_scheme":"Other"},{"subitem_subject":"Ceftazidime resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"bla(TEM-61)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare bla TEM-61","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare bla TEM-61","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-11-14"},"publish_date":"2022-11-14","publish_status":"0","recid":"2001238","relation_version_is_last":true,"title":["Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare bla TEM-61"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:42:45.182967+00:00"}