{"created":"2023-02-07T04:05:21.725310+00:00","id":2001290,"links":{},"metadata":{"_buckets":{"deposit":"883441f4-09b9-4b1d-89ff-7ead9292a609"},"_deposit":{"id":"2001290","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001290"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02001290","sets":["1016:1017"]},"author_link":[],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"24","bibliographicPageEnd":"10612","bibliographicPageStart":"10603","bibliographicVolumeNumber":"102","bibliographic_titles":[{"bibliographic_title":"Applied microbiology and biotechnology."}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have recently developed Corynebacterium glutamicum strains that produce free fatty acids in culture supernatant due to enhanced fatty acid biosynthesis. Of these producing strains, the basic producer PAS-15 has a defect in the gene for a fatty acid biosynthesis repressor protein, and the advanced producer PCC-6 has two additional mutations to augment the production by strain PAS-15. The aim of the present study was to obtain novel genetic traits for improving fatty acid production by these producers. A new mutant with increased production derived from strain PAS-15 had a missense mutation in the accD3 gene (mutation accD3A433T), which is involved in the biosynthesis of mycolic acids that are cell envelope lipids of C. glutamicum, as the causal mutation. Mutation accD3A433T was verified to reduce the AccD3 enzymatic activity and increase fatty acid production in strain PAS-15 by 1.8-fold. Deletion of the accD3 gene in strain PAS-15, which was motivated by the characteristic of mutation accD3A433T, increased fatty acid production by 3.2-fold. Susceptibility of strain PAS-15 to vancomycin was significantly increased by accD3 gene deletion and by mutation accD3A433T to the intermediate level, suggesting that the cell envelope permeability barrier by mycolic acids is weakened by this engineering. Furthermore, mutation accD3A433T also increased fatty acid production in strain PCC-6 by 1.3-fold. These increased production levels were suggested to be involved not only in the redirection of carbon flux from mycolic acid biosynthesis to fatty acid production but also in the permeability of the cell envelope.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Applied microbiology and biotechnology. 102(24) : 10603-10612(2018)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"竹野, 誠記","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html"},{"subitem_link_text":"池田, 正人","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer International"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"30276713"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/30276713/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1007/s00253-018-9395-5"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00253-018-9395-5","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00253-018-9395-5"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1432-0614","subitem_source_identifier_type":"EISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeno, Seiki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Murata, Noriko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kura, Moe","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takasaki, Manami","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hayashi, Mikiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ikeda, Masato","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-07"}],"filename":"ApplMicrobioBiotech102_10603.pdf","format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ApplMicrobioBiotech102_10603.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/2001290/files/ApplMicrobioBiotech102_10603.pdf"},"version_id":"d8e461b1-bf66-4beb-9b1c-58fc64aaf6d0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Corynebacterium glutamicum","subitem_subject_scheme":"Other"},{"subitem_subject":"fatty acid production","subitem_subject_scheme":"Other"},{"subitem_subject":"accD3","subitem_subject_scheme":"Other"},{"subitem_subject":"mycolic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"cell envelope permeability barrier","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-07"},"publish_date":"2023-02-07","publish_status":"0","recid":"2001290","relation_version_is_last":true,"title":["The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-02-07T04:05:25.909364+00:00"}