{"created":"2023-05-08T00:39:37.250519+00:00","id":2001447,"links":{},"metadata":{"_buckets":{"deposit":"45877ea5-de2a-4389-8fa6-1254c142523e"},"_deposit":{"id":"2001447","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001447"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:02001447","sets":["1016:1017"]},"author_link":[],"control_number":"2001447","item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-04-26","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographicVolumeNumber":"664","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications","bibliographic_titleLang":"en"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A myogenetic oligodeoxynucleotide, iSN04, is the 18-base single-stranded DNA that acts as an anti-nucleolin aptamer. iSN04 has been reported to restore myogenic differentiation by suppressing inflammatory responses in myoblasts isolated from patients with diabetes or healthy myoblasts exposed to cancer-releasing factors. Thus, iSN04 is expected to be a nucleic acid drug for the muscle wasting associated with chronic diseases. The present study investigated the anti-inflammatory mechanism of iSN04 in the murine myoblast cell line C2C12. Tumor necrosis factor-α (TNF-α) or Toll-like receptor (TLR) ligands (Pam3CSK4 and FSL-1) induced nuclear translocation and transcriptional activity of nuclear factor-κB (NF-κB), resulting in upregulated expression of TNF-α and interleukin-6. Pre-treatment with iSN04 significantly suppressed these inflammatory responses by inhibiting the nuclear accumulation of β-catenin induced by TNF-α or TLR ligands. These results demonstrate that antagonizing nucleolin with iSN04 downregulates the inflammatory effect mediated by the β-catenin/NF-κB signaling pathway in C2C12 cells. In addition, the anti-inflammatory effects of iSN04 were also observed in the rat smooth muscle cell line A10 and the murine adipocyte-like fibroblast cell line 3T3-L1, suggesting that iSN04 may be useful in preventing inflammation induced by metabolic disorders.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biochemical and biophysical research communications 664 : 1-8, (2023)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"三谷, 塁一","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html"},{"subitem_link_text":"高谷, 智英","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"37127012"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/37127012/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.bbrc.2023.04.098"}],"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2023.04.098","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1090-2104","subitem_source_identifier_type":"EISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamamoto, Machi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Miyoshi, Mana","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Morioka, Kamino","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mitani, Takakazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takaya, Tomohide","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-04-26"}],"filename":"BBRC664_1.pdf","format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"url":"https://soar-ir.repo.nii.ac.jp/record/2001447/files/BBRC664_1.pdf"},"version_id":"b1aee7fb-356b-490c-9604-decc9e2ee6a6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"β-catenin","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammatory response","subitem_subject_scheme":"Other"},{"subitem_subject":"Myoblast","subitem_subject_scheme":"Other"},{"subitem_subject":"Myogenetic oligodeoxynucleotide (myoDN)","subitem_subject_scheme":"Other"},{"subitem_subject":"Nuclear factor-kB (NF-kB)","subitem_subject_scheme":"Other"},{"subitem_subject":"Nucleolin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in C2C12 myoblasts by modulating the β-catenin/NF-κB signaling pathway","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in C2C12 myoblasts by modulating the β-catenin/NF-κB signaling pathway","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-05-08"},"publish_date":"2023-05-08","publish_status":"0","recid":"2001447","relation_version_is_last":true,"title":["Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in C2C12 myoblasts by modulating the β-catenin/NF-κB signaling pathway"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-08T01:17:59.209334+00:00"}