{"created":"2021-03-01T06:23:48.732264+00:00","id":20696,"links":{},"metadata":{"_buckets":{"deposit":"844ae914-d60b-40c1-aff4-82bdc0ef5e9a"},"_deposit":{"id":"20696","owners":[],"pid":{"revision_id":0,"type":"depid","value":"20696"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00020696","sets":["1016:1017"]},"author_link":["107972","107973","107974","107975","107976"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"1885","bibliographicPageStart":"1880","bibliographicVolumeNumber":"88","bibliographic_titles":[{"bibliographic_title":"ANIMAL SCIENCE JOURNAL"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"ANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Takaya, Tomohide","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"},{"subitem_link_text":"Ono, Tamao","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html"},{"subitem_link_text":"Kagami, Hiroshi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000414462000028"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"WILEY"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"28782148"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/28782148/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1111/asj.12884"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/asj.12884","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.<br/>"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344-3941","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11677214","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takaya, Tomohide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nihashi, Yuma"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kojima, Shotaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Tamao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kagami, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-05-08"}],"displaytype":"detail","filename":"2017_AnimSciJ_Takaya_SOAR.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensefree":"© 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.<br/>","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2017_AnimSciJ_Takaya_SOAR.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/20696/files/2017_AnimSciJ_Takaya_SOAR.pdf"},"version_id":"548e42fb-cdd4-4dec-995f-d402dee248fd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cell fusion","subitem_subject_scheme":"Other"},{"subitem_subject":"hybrid cell","subitem_subject_scheme":"Other"},{"subitem_subject":"myotube formation","subitem_subject_scheme":"Other"},{"subitem_subject":"skeletal muscle myoblast","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-05-08"},"publish_date":"2019-05-08","publish_status":"0","recid":"20696","relation_version_is_last":true,"title":["Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:30:31.621373+00:00"}