{"_buckets": {"deposit": "e9ce8088-091a-45e6-9e91-5e8f81207d84"}, "_deposit": {"id": "21116", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21116"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00021116"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-01-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageStart": "235", "bibliographicVolumeNumber": "19", "bibliographic_titles": [{"bibliographic_title": "INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The high affinity K+ transporter 1;4 (HKT1;4) in rice (Oryza sativa), which shows Na+ selective transport with little K+ transport activity, has been suggested to be involved in reducing Na in leaves and stems under salt stress. However, detailed physiological roles of OsHKT1;4 remain unknown. Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice. The homozygous mutant (the O/E line) accumulated significantly lower concentrations of Na in young leaves, stems, and seeds than the sibling WT line under salt stress. Interestingly, however, the mutation rendered the O/E plants more salt sensitive than WT plants. Together with the evaluation of biomass of rice lines, rhizosphere acidification assays using a pH indicator bromocresol purple and (NaCl)-Na-22 tracer experiments have led to an assumption that roots of O/E plants suffered heavier damages from Na which excessively accumulated in the root due to increased activity of Na+ uptake and Na+ exclusion in the vasculature. 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However, detailed physiological roles of OsHKT1;4 remain unknown. Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice. The homozygous mutant (the O/E line) accumulated significantly lower concentrations of Na in young leaves, stems, and seeds than the sibling WT line under salt stress. Interestingly, however, the mutation rendered the O/E plants more salt sensitive than WT plants. Together with the evaluation of biomass of rice lines, rhizosphere acidification assays using a pH indicator bromocresol purple and (NaCl)-Na-22 tracer experiments have led to an assumption that roots of O/E plants suffered heavier damages from Na which excessively accumulated in the root due to increased activity of Na+ uptake and Na+ exclusion in the vasculature. Implications toward the application of the HKT1-mediated Na+ exclusion system to the breeding of salt tolerant crop cultivars will be discussed."}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "salt stress; high affinity K+ transporter (HKT); rice; Na+ exclusion"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Oda, Yuuka"}], "nameIdentifiers": [{"nameIdentifier": "108990", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Natsuko I."}], "nameIdentifiers": [{"nameIdentifier": "108991", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanoi, Keitaro"}], "nameIdentifiers": [{"nameIdentifier": "108992", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ma, Jian Feng"}], "nameIdentifiers": [{"nameIdentifier": "108993", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itou, Yukiko"}], "nameIdentifiers": [{"nameIdentifier": "108994", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katsuhara, Maki"}], "nameIdentifiers": [{"nameIdentifier": "108995", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itou, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "108996", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Horie, Tomoaki"}], "nameIdentifiers": [{"nameIdentifier": "108997", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-02-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "15K07810_01.pdf", "filesize": [{"value": "10.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "\u00a9 2018 by the authors. 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