@article{oai:soar-ir.repo.nii.ac.jp:00021116,
 author = {Oda, Yuuka and Kobayashi, Natsuko I. and Tanoi, Keitaro and Ma, Jian Feng and Itou, Yukiko and Katsuhara, Maki and Itou, Takashi and Horie, Tomoaki},
 issue = {1},
 journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
 month = {Jan},
 note = {The high affinity K+ transporter 1;4 (HKT1;4) in rice (Oryza sativa), which shows Na+ selective transport with little K+ transport activity, has been suggested to be involved in reducing Na in leaves and stems under salt stress. However, detailed physiological roles of OsHKT1;4 remain unknown. Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice. The homozygous mutant (the O/E line) accumulated significantly lower concentrations of Na in young leaves, stems, and seeds than the sibling WT line under salt stress. Interestingly, however, the mutation rendered the O/E plants more salt sensitive than WT plants. Together with the evaluation of biomass of rice lines, rhizosphere acidification assays using a pH indicator bromocresol purple and (NaCl)-Na-22 tracer experiments have led to an assumption that roots of O/E plants suffered heavier damages from Na which excessively accumulated in the root due to increased activity of Na+ uptake and Na+ exclusion in the vasculature. Implications toward the application of the HKT1-mediated Na+ exclusion system to the breeding of salt tolerant crop cultivars will be discussed., Article, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.19(1):235(2018)},
 title = {T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress},
 volume = {19},
 year = {2018}
}