{"created":"2021-03-01T06:24:15.075408+00:00","id":21123,"links":{},"metadata":{"_buckets":{"deposit":"198660a0-b6aa-4af3-bf44-686a1fa1cbc9"},"_deposit":{"id":"21123","owners":[],"pid":{"revision_id":0,"type":"depid","value":"21123"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00021123","sets":["1016:1017"]},"author_link":["109031","109032","109033","109034","109035"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"23980","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (> 90%) mutagenized in cultured chicken primordial germ cells (PGCs) by transfection of circular plasmids encoding Cas9, a single guide RNA, and a gene encoding drug resistance, followed by transient antibiotic selection. We transplanted CRISPR-induced mutant-ovomucoid PGCs into recipient chicken embryos and established three germline chimeric roosters (G0). All of the roosters had donor-derived mutant-ovomucoid spermatozoa, and the two with a high transmission rate of donor-derived gametes produced heterozygous mutant ovomucoid chickens as about half of their donor-derived offspring in the next generation (G1). Furthermore, we generated ovomucoid homozygous mutant offspring (G2) by crossing the G1 mutant chickens. Taken together, these results demonstrate that the CRISPR/Cas9 system is a simple and effective gene-targeting method in chickens.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"SCIENTIFIC REPORTS.6:23980(2016)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Miyahara, Daichi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.uhfmbCkF.html"},{"subitem_link_text":"Kagami, Hiroshi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_text":"Web of Science","subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000373432200001"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"NATURE PUBLISHING GROUP"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"27050479"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/27050479/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/srep23980"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep23980","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Oishi, Isao"}],"nameIdentifiers":[{"nameIdentifier":"109031","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshii, Kyoko"}],"nameIdentifiers":[{"nameIdentifier":"109032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyahara, Daichi"}],"nameIdentifiers":[{"nameIdentifier":"109033","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kagami, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"109034","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tagami, Takahiro"}],"nameIdentifiers":[{"nameIdentifier":"109035","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-28"}],"displaytype":"detail","filename":"26450376_03.pdf","filesize":[{"value":"6.8 MB"}],"format":"application/pdf","licensefree":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26450376_03.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/21123/files/26450376_03.pdf"},"version_id":"35d95e71-d34d-4d4f-9f07-6bc13f74d099"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Agricultural genetics","subitem_subject_scheme":"Other"},{"subitem_subject":"Genetic engineering","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Targeted mutagenesis in chicken using CRISPR/Cas9 system","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Targeted mutagenesis in chicken using CRISPR/Cas9 system","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-02-28"},"publish_date":"2020-02-28","publish_status":"0","recid":"21123","relation_version_is_last":true,"title":["Targeted mutagenesis in chicken using CRISPR/Cas9 system"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:18:38.829427+00:00"}