{"_buckets": {"deposit": "54986905-8311-4e50-a7c0-f1eb9cab262b"}, "_deposit": {"id": "21348", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21348"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00021348"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-02-04", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "768", "bibliographicPageStart": "758", "bibliographicVolumeNumber": "105", "bibliographic_titles": [{"bibliographic_title": "INTERNATIONAL JOURNAL OF HEMATOLOGY"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Fibrinogen storage disease (FSD) is a rare disorder that is characterized by the accumulation of fibrinogen in hepatocytes and induces liver injury. Six mutations in the \u03b3C domain (\u03b3G284R, \u03b3T314P, \u03b3D316N, the deletion of \u03b3G346-Q350, \u03b3G366S, and \u03b3R375W) have been identified for FSD. Our group previously established \u03b3375W fibrinogen-producing Chinese hamster ovary (CHO) cells and observed aberrant large granular and fibrous forms of intracellular inclusion bodies. The aim of this study was to investigate whether fibrous intracellular inclusion bodies are specific to FSD-inducible variant fibrinogen. Thirteen expression vectors encoding the variant \u03b3-chain were stably or transiently transfected into CHO cells expressing normal fibrinogen A\u03b1- and B\u03b2-chains or HuH-7 cells, which were then immunofluorescently stained. Six CHO and HuH-7 cell lines that transiently produced FSD-inducible variant fibrinogen presented the fibrous (3.2\u201322.7 and 2.1\u201324.5%, respectively) and large granular (5.4\u201325.5 and 7.7\u201323.9%) forms of intracellular inclusion bodies. Seven CHO and HuH-7 cell lines that transiently produced FSD-non-inducible variant fibrinogen only exhibit the large granular form. These results demonstrate that transiently transfected variant fibrinogen-producing CHO cells and inclusion bodies of the fibrous form may be useful in non-invasive screening for FSD risk factors for FSD before its onset.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "INTERNATIONAL JOURNAL OF HEMATOLOGY.105:758-768(2017)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Arai, Shinpei", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.uCnFPUym.html"}, {"subitem_link_text": "Honda, Takayuki", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.WeDUHFkh.html"}, {"subitem_link_text": "Okumura, Nobuo", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000401324100008"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "SPRINGER JAPAN KK"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "28161763"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.ncbi.nlm.nih.gov/pubmed/28161763", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s12185-017-2185-5"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s12185-017-2185-5", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This is a post-peer-review, pre-copyedit version of an article published in INTERNATIONAL JOURNAL OF HEMATOLOGY. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12185-017-2185-5"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0925-5710", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0925-5710", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10797094", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Intracellular inclusion bodies; Hypofibrinogenemia; Fibrinogen storage disease; Immunofluorescence; Recombinant fibrinogen"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "ENDOPLASMIC-RETICULUM-STORAGE; LIVER-DISEASE; ALPHA(1)-ANTITRYPSIN DEFICIENCY; C-TERMINUS; MUTATION; HYPOFIBRINOGENEMIA; GAMMA; AGUADILLA; HYPODYSFIBRINOGENEMIA; POLYMERIZATION"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Arai, Shinpei"}], "nameIdentifiers": [{"nameIdentifier": "109380", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogiwara, Naoko"}], "nameIdentifiers": [{"nameIdentifier": "109381", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mukai, Saki"}], "nameIdentifiers": [{"nameIdentifier": "109382", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takezawa, Yuka"}], "nameIdentifiers": [{"nameIdentifier": "109383", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugano, Mitsutoshi"}], "nameIdentifiers": [{"nameIdentifier": "109384", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Honda, Takayuki"}], "nameIdentifiers": [{"nameIdentifier": "109385", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okumura, Nobuo"}], "nameIdentifiers": [{"nameIdentifier": "109386", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-05-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "INTERNATIONALJOURNALOFHEMATOLOGY105_758.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This is a post-peer-review, pre-copyedit version of an article published in INTERNATIONAL JOURNAL OF HEMATOLOGY. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12185-017-2185-5", "licensetype": "license_free", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "INTERNATIONALJOURNALOFHEMATOLOGY105_758.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/21348/files/INTERNATIONALJOURNALOFHEMATOLOGY105_758.pdf"}, "version_id": "6b86a6c1-0f0f-40c3-849c-5be8645b9f55"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Intracellular inclusion bodies", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Hypofibrinogenemia", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fibrinogen storage disease", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Immunofluorescence", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Recombinant fibrinogen", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The fibrous form of intracellular inclusion bodies in recombinant variant fibrinogen-producing cells is specific to the hepatic fibrinogen storage disease-inducible variant fibrinogen", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "The fibrous form of intracellular inclusion bodies in recombinant variant fibrinogen-producing cells is specific to the hepatic fibrinogen storage disease-inducible variant fibrinogen"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/00022105", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2020-05-15"}, "publish_date": "2020-05-15", "publish_status": "0", "recid": "21348", "relation": {}, "relation_version_is_last": true, "title": ["The fibrous form of intracellular inclusion bodies in recombinant variant fibrinogen-producing cells is specific to the hepatic fibrinogen storage disease-inducible variant fibrinogen"], "weko_shared_id": null}