{"created":"2021-03-01T06:24:36.461543+00:00","id":21464,"links":{},"metadata":{"_buckets":{"deposit":"86b36496-ecbf-4b6d-843f-55f692c96412"},"_deposit":{"id":"21464","owners":[],"pid":{"revision_id":0,"type":"depid","value":"21464"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00021464","sets":["1016:1017"]},"author_link":["109640","109641","109642","109643","109644","109645","109646","109647","109648","109649"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-16","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"579","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Frontiers in Pharmacology"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Wooden breast syndrome (WB) constitutes an emerging myopathy in the pectoralis major muscle (PM) of broiler chickens, characterized by myofiber hypertrophy and degeneration along with severe fibrosis. WB pathogenesis has been considered to involve hypoxia induced by rapid growth of the PM. In this study, we focused on mitochondrial morphology and dynamics in the myofibers, as these organelles are sensitive to damage by hypoxia, and examined the effects on WB pathogenesis. Specifically, the PMs of a flock of 35 broilers at 50 days of age were evaluated. First, the severity of disease in each bird was determined by measuring histopathological indices including the fibrotic area (FA) in the muscle and circularity of myofibers (CM). These values were 29.4 ± 9.6% and 0.70 ± 0.042, respectively, showing variety among the flock. Myofiber vacuolization was observed in all birds including numerous small- or large-rimmed vacuoles, with the former consisting of ultrastructurally autophagosome-like vacuoles engulfing degenerated mitochondria. The large-rimmed vacuoles frequently occurred in the PMs with more severe FA and CM, indicating a relationship between altered autophagy/mitophagy and WB severity. Next, the expression levels of hypoxia-adaptive and mitochondrial dynamics-related genes were analyzed, and their correlations with the histopathological indices were examined. The histopathological indices were negatively correlated with the expression of vascular endothelial growth factor A (VEGFA), indicating that less angiogenesis owing to weakened hypoxia-inducible factor signaling induces more severe WB pathology. In addition, the observed negative correlation with mitochondrial dynamics-related genes implied that WB pathology deteriorates concomitant with reduced mitochondrial dynamics. Furthermore, the expression of mitochondrial dynamics-related genes showed strong positive correlation with that of VEGFA and autophagy-/mitophagy-related genes. These results revealed that the PMs of broilers possess the mechanism of physiological clearance of mitochondria damaged by the hypoxia resulting from the continuous mitochondrial dynamics and autophagy/mitophagy accompanying rapid PM growth. In turn, the altered mitochondrial clearance induced by chronic hypoxia and the accumulation of damaged mitochondria likely underly the severe pathological features of WB.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Frontiers in Pharmacology.11:579(2020)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Takaya, Tomohide","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3389/fphys.2020.00579"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3389/fphys.2020.00579","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 Hosotani, Kawasaki, Hasegawa, Wakasa, Hoshino, Takahashi, Ueda, Takaya, Iwasaki and Watanabe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1663-9812","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hosotani, Marina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawasaki, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasegawa, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wakasa, Yui"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hoshino, Maki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueda, Hiromi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaya, Tomohide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwasaki, Tomohito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Takafumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-22"}],"displaytype":"detail","filename":"2020_FrontPhysiol_11_579.pdf","filesize":[{"value":"4.1 MB"}],"format":"application/pdf","licensefree":"© 2020 Hosotani, Kawasaki, Hasegawa, Wakasa, Hoshino, Takahashi, Ueda, Takaya, Iwasaki and Watanabe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2020_FrontPhysiol_11_579.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/21464/files/2020_FrontPhysiol_11_579.pdf"},"version_id":"915577d8-e12b-4bd7-b639-f70f97b47dff"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"wooden breast syndrome","subitem_subject_scheme":"Other"},{"subitem_subject":"pathology","subitem_subject_scheme":"Other"},{"subitem_subject":"hypoxia","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondria","subitem_subject_scheme":"Other"},{"subitem_subject":"autophagy","subitem_subject_scheme":"Other"},{"subitem_subject":"mitophagy","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-22"},"publish_date":"2020-06-22","publish_status":"0","recid":"21464","relation_version_is_last":true,"title":["Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:19:14.879126+00:00"}