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Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin
http://hdl.handle.net/10091/00022442
http://hdl.handle.net/10091/000224428296e324-e026-453b-9e4f-2a58420f7815
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-01-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | oligodeoxynucleotide (ODN) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | berberine (CID: 12457) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nucleolin (NCL) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | skeletal muscle myoblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | myogenic differentiation | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Shinji, Sayaka
× Shinji, Sayaka× Umezawa, Koji× Nihashi, Yuma× Nakamura, Shunichi× Shimosato, Takeshi× Takaya, Tomohide |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Umezawa, Koji | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yVcFPhkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosato, Takeshi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Takaya, Tomohide | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html | |||||
出版者 | ||||||
出版者 | FRONTIERS MEDIA SA | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 8:616706 (2021) | |||||
書誌情報 |
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 巻 8, p. 616706, 発行日 2021-01-11 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Herein we report that the 18-base telomeric oligodeoxynucleotides (ODNs) designed from the Lactobacillus rhamnosus GG genome promote differentiation of skeletal muscle myoblasts which are myogenic precursor cells. We termed these myogenetic ODNs (myoDNs). The activity of one of the myoDNs, iSN04, was independent of Toll-like receptors, but dependent on its conformational state. Molecular simulation and iSN04 mutants revealed stacking of the 13–15th guanines as a core structure for iSN04. The alkaloid berberine bound to the guanine stack and enhanced iSN04 activity, probably by stabilizing and optimizing iSN04 conformation. We further identified nucleolin as an iSN04-binding protein. Results showed that iSN04 antagonizes nucleolin, increases the levels of p53 protein translationally suppressed by nucleolin, and eventually induces myotube formation by modulating the expression of genes involved in myogenic differentiation and cell cycle arrest. This study shows that bacterial-derived myoDNs serve as aptamers and are potential nucleic acid drugs directly targeting myoblasts. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2296-634X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3389/fcell.2020.616706 | |||||
関連名称 | 10.3389/fcell.2020.616706 | |||||
権利 | ||||||
権利情報 | Copyright © 2021 Shinji, Umezawa, Nihashi, Nakamura, Shimosato and Takaya.This is an open-access article distributed under the terms of the Creative CommonsAttribution License (CC BY). The use, distribution or reproduction in other forumsis permitted, provided the original author(s) and the copyright owner(s) are creditedand that the original publication in this journal is cited, in accordance with acceptedacademic practice. No use, distribution or reproduction is permitted which does notcomply with these terms. | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |