{"created":"2021-03-01T06:24:50.308089+00:00","id":21685,"links":{},"metadata":{"_buckets":{"deposit":"fa845b97-52f5-4788-a660-e425a068e784"},"_deposit":{"id":"21685","owners":[],"pid":{"revision_id":0,"type":"depid","value":"21685"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00021685","sets":["1016:1017"]},"author_link":["110007","110008","110009","110010","110011","110012"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-01-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"616706","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Herein we report that the 18-base telomeric oligodeoxynucleotides (ODNs) designed from the Lactobacillus rhamnosus GG genome promote differentiation of skeletal muscle myoblasts which are myogenic precursor cells. We termed these myogenetic ODNs (myoDNs). The activity of one of the myoDNs, iSN04, was independent of Toll-like receptors, but dependent on its conformational state. Molecular simulation and iSN04 mutants revealed stacking of the 13–15th guanines as a core structure for iSN04. The alkaloid berberine bound to the guanine stack and enhanced iSN04 activity, probably by stabilizing and optimizing iSN04 conformation. We further identified nucleolin as an iSN04-binding protein. Results showed that iSN04 antagonizes nucleolin, increases the levels of p53 protein translationally suppressed by nucleolin, and eventually induces myotube formation by modulating the expression of genes involved in myogenic differentiation and cell cycle arrest. This study shows that bacterial-derived myoDNs serve as aptamers and are potential nucleic acid drugs directly targeting myoblasts.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 8:616706 (2021)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Umezawa, Koji","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.yVcFPhkh.html"},{"subitem_link_text":"Shimosato, Takeshi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"},{"subitem_link_text":"Takaya, Tomohide","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"FRONTIERS MEDIA SA"}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3389/fcell.2020.616706"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3389/fcell.2020.616706","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2021 Shinji, Umezawa, Nihashi, Nakamura, Shimosato and Takaya.This is an open-access article distributed under the terms of the Creative CommonsAttribution License (CC BY). The use, distribution or reproduction in other forumsis permitted, provided the original author(s) and the copyright owner(s) are creditedand that the original publication in this journal is cited, in accordance with acceptedacademic practice. No use, distribution or reproduction is permitted which does notcomply with these terms."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2296-634X","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shinji, Sayaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Umezawa, Koji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nihashi, Yuma"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Shunichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosato, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaya, Tomohide"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-01-12"}],"displaytype":"detail","filename":"2021_FrontCellDevBiol_Shinji.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"2021_FrontCellDevBiol_Shinji.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/21685/files/2021_FrontCellDevBiol_Shinji.pdf"},"version_id":"9d1b43f9-7712-46c6-8e7b-59821a6253ae"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"oligodeoxynucleotide (ODN)","subitem_subject_scheme":"Other"},{"subitem_subject":"berberine (CID: 12457)","subitem_subject_scheme":"Other"},{"subitem_subject":"nucleolin (NCL)","subitem_subject_scheme":"Other"},{"subitem_subject":"skeletal muscle myoblast","subitem_subject_scheme":"Other"},{"subitem_subject":"myogenic differentiation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-01-12"},"publish_date":"2021-01-12","publish_status":"0","recid":"21685","relation_version_is_last":true,"title":["Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:19:33.453432+00:00"}