WEKO3
アイテム
{"_buckets": {"deposit": "3118fd78-dc69-41d2-aa64-a8a523aec88a"}, "_deposit": {"id": "21756", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21756"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00021756", "sets": ["462"]}, "author_link": ["110243", "110244", "110245", "110246", "110247", "110248", "110249", "110250"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-09-15", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageStart": "e0162693", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "PLOS ONE"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are potential resources for the regeneration of defective organs, including the liver. However, some obstaclesmust be overcome before this becomes reality. Undifferentiated cells that remain following differentiation have teratoma-forming potential. Additionally, practical applications require a large quantity of differentiated cells, so the differentiation processmust be economical. Here we describe a DNA microarray-based global analysis of the gene expression profiles of differentiating human pluripotent stem cells. We identified differences and commonalities among six human pluripotent stem cell lines: the hESCs KhES1, KhES2, KhES3, and H1, and the iPSCs 201B7 and 243G1. Embryoid bodies (EBs) formed without requiring supplementation with inducing factors. EBs also expressed some liver-specific metabolic genes including the ammonia-metabolizing enzymes glutamine synthetase and carbamoyl-phosphate synthase 1. Real-time PCR analysis revealed hepatocyte-like differentiation of EBs treated with ammonia in Lanford medium. Analysis of DNA microarray data suggested that hepatocyte-like cells were the most abundant population in ammonia-treated cells. Furthermore, expression levels of undifferentiated pluripotent stem cell markers were drastically reduced, suggesting a reduced teratoma-forming capacity. These results indicate that treatment of EBs with ammonia in Lanford medium may be an effective inducer of hepatic differentiation in absence of expensive inducing factors.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ(コンテンツの種類)", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "PLOS ONE.11(9):e0162693(2016)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Tomotsune, Daihachiro", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html"}, {"subitem_link_text": "Yue, Fengming", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.jCLmuNkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000383706900065"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "PUBLIC LIBRARY SCIENCE"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "27632182"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.ncbi.nlm.nih.gov/pubmed/27632182", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1371/journal.pone.0162693"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1371/journal.pone.0162693", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2016 Tomotsune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "EISSN"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "HUMAN ES CELLS; GLUTAMINE-SYNTHETASE; MOUSE; LIVER; DIFFERENTIATION; EXPRESSION; COLONIES; CULTURE"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are potential resources for the regeneration of defective organs, including the liver. However, some obstaclesmust be overcome before this becomes reality. Undifferentiated cells that remain following differentiation have teratoma-forming potential. Additionally, practical applications require a large quantity of differentiated cells, so the differentiation processmust be economical. Here we describe a DNA microarray-based global analysis of the gene expression profiles of differentiating human pluripotent stem cells. We identified differences and commonalities among six human pluripotent stem cell lines: the hESCs KhES1, KhES2, KhES3, and H1, and the iPSCs 201B7 and 243G1. Embryoid bodies (EBs) formed without requiring supplementation with inducing factors. EBs also expressed some liver-specific metabolic genes including the ammonia-metabolizing enzymes glutamine synthetase and carbamoyl-phosphate synthase 1. Real-time PCR analysis revealed hepatocyte-like differentiation of EBs treated with ammonia in Lanford medium. Analysis of DNA microarray data suggested that hepatocyte-like cells were the most abundant population in ammonia-treated cells. Furthermore, expression levels of undifferentiated pluripotent stem cell markers were drastically reduced, suggesting a reduced teratoma-forming capacity. These results indicate that treatment of EBs with ammonia in Lanford medium may be an effective inducer of hepatic differentiation in absence of expensive inducing factors."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tomotsune, Daihachiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110243", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirashima, Kanji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110244", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujii, Masako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110245", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yue, Fengming", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110246", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Ken", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110247", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takizawa-Shirasawa, Sakiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110248", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokoyama, Tadayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110249", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sasaki, Katsunori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "110250", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-02-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "15K01280_01.pdf", "filesize": [{"value": "3.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "© 2016 Tomotsune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.", "licensetype": "license_note", "mimetype": "application/pdf", "size": 3200000.0, "url": {"label": "15K01280_01.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/21756/files/15K01280_01.pdf"}, "version_id": "b2f00cab-fb74-4ed5-a147-69b6aa19bd13"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/00022513", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2021-02-15"}, "publish_date": "2021-02-15", "publish_status": "0", "recid": "21756", "relation": {}, "relation_version_is_last": true, "title": ["Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment"], "weko_shared_id": -1}
Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment
http://hdl.handle.net/10091/00022513
http://hdl.handle.net/10091/0002251340c13aa9-8af3-447d-a4fc-33a1cb271ef1
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2021-02-15 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Tomotsune, Daihachiro
× Tomotsune, Daihachiro× Hirashima, Kanji× Fujii, Masako× Yue, Fengming× Matsumoto, Ken× Takizawa-Shirasawa, Sakiko× Yokoyama, Tadayuki× Sasaki, Katsunori |
|||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Tomotsune, Daihachiro | |||||
URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Yue, Fengming | |||||
URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.jCLmuNkh.html | |||||
出版者 | ||||||
出版者 | PUBLIC LIBRARY SCIENCE | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLOS ONE.11(9):e0162693(2016) | |||||
書誌情報 |
PLOS ONE 巻 11, 号 9, p. e0162693, 発行日 2016-09-15 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are potential resources for the regeneration of defective organs, including the liver. However, some obstaclesmust be overcome before this becomes reality. Undifferentiated cells that remain following differentiation have teratoma-forming potential. Additionally, practical applications require a large quantity of differentiated cells, so the differentiation processmust be economical. Here we describe a DNA microarray-based global analysis of the gene expression profiles of differentiating human pluripotent stem cells. We identified differences and commonalities among six human pluripotent stem cell lines: the hESCs KhES1, KhES2, KhES3, and H1, and the iPSCs 201B7 and 243G1. Embryoid bodies (EBs) formed without requiring supplementation with inducing factors. EBs also expressed some liver-specific metabolic genes including the ammonia-metabolizing enzymes glutamine synthetase and carbamoyl-phosphate synthase 1. Real-time PCR analysis revealed hepatocyte-like differentiation of EBs treated with ammonia in Lanford medium. Analysis of DNA microarray data suggested that hepatocyte-like cells were the most abundant population in ammonia-treated cells. Furthermore, expression levels of undifferentiated pluripotent stem cell markers were drastically reduced, suggesting a reduced teratoma-forming capacity. These results indicate that treatment of EBs with ammonia in Lanford medium may be an effective inducer of hepatic differentiation in absence of expensive inducing factors. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 1932-6203 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://www.ncbi.nlm.nih.gov/pubmed/27632182 | |||||
関連名称 | 27632182 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1371/journal.pone.0162693 | |||||
関連名称 | 10.1371/journal.pone.0162693 | |||||
権利 | ||||||
権利情報 | © 2016 Tomotsune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000383706900065 |