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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment

http://hdl.handle.net/10091/00022513
http://hdl.handle.net/10091/00022513
40c13aa9-8af3-447d-a4fc-33a1cb271ef1
名前 / ファイル ライセンス アクション
15K01280_01.pdf 15K01280_01.pdf (3.2 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2021-02-15
タイトル
言語 en
タイトル Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Tomotsune, Daihachiro

× Tomotsune, Daihachiro

WEKO 110243

en Tomotsune, Daihachiro

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Hirashima, Kanji

× Hirashima, Kanji

WEKO 110244

en Hirashima, Kanji

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Fujii, Masako

× Fujii, Masako

WEKO 110245

en Fujii, Masako

Search repository
Yue, Fengming

× Yue, Fengming

WEKO 110246

en Yue, Fengming

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Matsumoto, Ken

× Matsumoto, Ken

WEKO 110247

en Matsumoto, Ken

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Takizawa-Shirasawa, Sakiko

× Takizawa-Shirasawa, Sakiko

WEKO 110248

en Takizawa-Shirasawa, Sakiko

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Yokoyama, Tadayuki

× Yokoyama, Tadayuki

WEKO 110249

en Yokoyama, Tadayuki

Search repository
Sasaki, Katsunori

× Sasaki, Katsunori

WEKO 110250

en Sasaki, Katsunori

Search repository
信州大学研究者総覧へのリンク
氏名 Tomotsune, Daihachiro
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html
信州大学研究者総覧へのリンク
氏名 Yue, Fengming
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.jCLmuNkh.html
出版者
出版者 PUBLIC LIBRARY SCIENCE
引用
内容記述タイプ Other
内容記述 PLOS ONE.11(9):e0162693(2016)
書誌情報 PLOS ONE

巻 11, 号 9, p. e0162693, 発行日 2016-09-15
抄録
内容記述タイプ Abstract
内容記述 Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are potential resources for the regeneration of defective organs, including the liver. However, some obstaclesmust be overcome before this becomes reality. Undifferentiated cells that remain following differentiation have teratoma-forming potential. Additionally, practical applications require a large quantity of differentiated cells, so the differentiation processmust be economical. Here we describe a DNA microarray-based global analysis of the gene expression profiles of differentiating human pluripotent stem cells. We identified differences and commonalities among six human pluripotent stem cell lines: the hESCs KhES1, KhES2, KhES3, and H1, and the iPSCs 201B7 and 243G1. Embryoid bodies (EBs) formed without requiring supplementation with inducing factors. EBs also expressed some liver-specific metabolic genes including the ammonia-metabolizing enzymes glutamine synthetase and carbamoyl-phosphate synthase 1. Real-time PCR analysis revealed hepatocyte-like differentiation of EBs treated with ammonia in Lanford medium. Analysis of DNA microarray data suggested that hepatocyte-like cells were the most abundant population in ammonia-treated cells. Furthermore, expression levels of undifferentiated pluripotent stem cell markers were drastically reduced, suggesting a reduced teratoma-forming capacity. These results indicate that treatment of EBs with ammonia in Lanford medium may be an effective inducer of hepatic differentiation in absence of expensive inducing factors.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ EISSN
収録物識別子 1932-6203
PubMed
識別子タイプ PMID
関連識別子 https://www.ncbi.nlm.nih.gov/pubmed/27632182
関連名称 27632182
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1371/journal.pone.0162693
関連名称 10.1371/journal.pone.0162693
権利
権利情報 © 2016 Tomotsune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000383706900065
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