{"created":"2021-03-01T06:24:54.754522+00:00","id":21756,"links":{},"metadata":{"_buckets":{"deposit":"3118fd78-dc69-41d2-aa64-a8a523aec88a"},"_deposit":{"id":"21756","owners":[],"pid":{"revision_id":0,"type":"depid","value":"21756"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00021756","sets":["461:462"]},"author_link":["110243","110244","110245","110246","110247","110248","110249","110250"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageStart":"e0162693","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are potential resources for the regeneration of defective organs, including the liver. However, some obstaclesmust be overcome before this becomes reality. Undifferentiated cells that remain following differentiation have teratoma-forming potential. Additionally, practical applications require a large quantity of differentiated cells, so the differentiation processmust be economical. Here we describe a DNA microarray-based global analysis of the gene expression profiles of differentiating human pluripotent stem cells. We identified differences and commonalities among six human pluripotent stem cell lines: the hESCs KhES1, KhES2, KhES3, and H1, and the iPSCs 201B7 and 243G1. Embryoid bodies (EBs) formed without requiring supplementation with inducing factors. EBs also expressed some liver-specific metabolic genes including the ammonia-metabolizing enzymes glutamine synthetase and carbamoyl-phosphate synthase 1. Real-time PCR analysis revealed hepatocyte-like differentiation of EBs treated with ammonia in Lanford medium. Analysis of DNA microarray data suggested that hepatocyte-like cells were the most abundant population in ammonia-treated cells. Furthermore, expression levels of undifferentiated pluripotent stem cell markers were drastically reduced, suggesting a reduced teratoma-forming capacity. These results indicate that treatment of EBs with ammonia in Lanford medium may be an effective inducer of hepatic differentiation in absence of expensive inducing factors.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLOS ONE.11(9):e0162693(2016)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Tomotsune, Daihachiro","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html"},{"subitem_link_text":"Yue, Fengming","subitem_link_url":"https://soar-rd.shinshu-u.ac.jp/profile/ja.jCLmuNkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_text":"Web of Science","subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000383706900065"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PUBLIC LIBRARY SCIENCE"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"27632182"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.ncbi.nlm.nih.gov/pubmed/27632182","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1371/journal.pone.0162693"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1371/journal.pone.0162693","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016 Tomotsune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"EISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tomotsune, Daihachiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirashima, Kanji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujii, Masako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yue, Fengming","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takizawa-Shirasawa, Sakiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoyama, Tadayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki, Katsunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"110250","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-15"}],"displaytype":"detail","filename":"15K01280_01.pdf","filesize":[{"value":"3.2 MB"}],"format":"application/pdf","licensefree":"© 2016 Tomotsune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15K01280_01.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/21756/files/15K01280_01.pdf"},"version_id":"b2f00cab-fb74-4ed5-a147-69b6aa19bd13"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-15"},"publish_date":"2021-02-15","publish_status":"0","recid":"21756","relation_version_is_last":true,"title":["Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:19:38.995730+00:00"}