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Human monoblastic leukemia cells (U937) were exposed to As-grown MWCNTs and MWCNTs that were thermally treated at 1800 degrees C (HTT1800) and 2800 degrees C (HTT2800). Cell proliferation was highly inhibited by As-grown but not HTT2800. However, both As-grown and HTT1800, which include some impurities, were cytotoxic. Proteomics analysis of MWCNT-exposed cells revealed 37 protein spots on 2-dimensional electrophoresis gels that significantly changed (p\u003c0.05) after exposure to HTT1800 with a little iron and 20 spots that changed after exposure to HTT2800. Peptide mass fingerprinting identified 45 proteins that included heat shock protein beta-1, neutral alpha-glucosidase AB, and DNA mismatch repair protein Msh2. These altered proteins play roles in metabolism, biosynthesis, response to stress, and cell differentiation. Although HTT2800 did not inhibit cell proliferation or cause cytotoxicity in vitro, some proteins related to the response to stress were changed. Moreover, DJ-1 protein, which is a biomarker of Parkinson\u0027s disease and is related to cancer, was identified after exposure to both MWCNTs. These results show that the cytotoxicity of MWCNTs depends on their impurities, such as iron, while MWCNTs themselves cause some biological responses directly and/or indirectly in vitro. Our proteomics-based approach for detecting biological responses to nanomaterials is a promising new method for detailed safety evaluations. (C) 2009 Elsevier Inc. 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Proteomics-based safety evaluation of multi-walled carbon nanotubes
http://hdl.handle.net/10091/10727
http://hdl.handle.net/10091/10727344422d9-90cd-44a6-bd5d-249f8ee0ea7a
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-10-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Proteomics-based safety evaluation of multi-walled carbon nanotubes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Carbon nanotubes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Safety evaluation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Proteomics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In vitro | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Haniu, Hisao
× Haniu, Hisao× Matsuda, Yoshikazu× Takeuchi, Kenji× Kim, Yoong Ahm× Hayashi, Takuya× Endo, Morinobu |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Haniu, Hisao | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Hayashi, Takuya | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jmLUbpkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Endo, Morinobu | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uUTmZhkh.html | |||||
出版者 | ||||||
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Toxicology and Applied Pharmacology. 242(3):256-262 (2010) | |||||
書誌情報 |
Toxicology and Applied Pharmacology 巻 242, 号 3, p. 256-262, 発行日 2010-02-01 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study evaluated the biological responses to multi-walled carbon nanotubes (MWCNTs). Human monoblastic leukemia cells (U937) were exposed to As-grown MWCNTs and MWCNTs that were thermally treated at 1800 degrees C (HTT1800) and 2800 degrees C (HTT2800). Cell proliferation was highly inhibited by As-grown but not HTT2800. However, both As-grown and HTT1800, which include some impurities, were cytotoxic. Proteomics analysis of MWCNT-exposed cells revealed 37 protein spots on 2-dimensional electrophoresis gels that significantly changed (p<0.05) after exposure to HTT1800 with a little iron and 20 spots that changed after exposure to HTT2800. Peptide mass fingerprinting identified 45 proteins that included heat shock protein beta-1, neutral alpha-glucosidase AB, and DNA mismatch repair protein Msh2. These altered proteins play roles in metabolism, biosynthesis, response to stress, and cell differentiation. Although HTT2800 did not inhibit cell proliferation or cause cytotoxicity in vitro, some proteins related to the response to stress were changed. Moreover, DJ-1 protein, which is a biomarker of Parkinson's disease and is related to cancer, was identified after exposure to both MWCNTs. These results show that the cytotoxicity of MWCNTs depends on their impurities, such as iron, while MWCNTs themselves cause some biological responses directly and/or indirectly in vitro. Our proteomics-based approach for detecting biological responses to nanomaterials is a promising new method for detailed safety evaluations. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0041-008X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00864435 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/19874835/ | |||||
関連名称 | 19874835 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.taap.2009.10.015 | |||||
関連名称 | 10.1016/j.taap.2009.10.015 | |||||
権利 | ||||||
権利情報 | Copyright © 2009 Elsevier Inc. | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000273832500003 |