{"created":"2021-03-01T06:07:30.661448+00:00","id":3679,"links":{},"metadata":{"_buckets":{"deposit":"33deac1c-456e-4126-a5d6-6875461e9f2c"},"_deposit":{"id":"3679","owners":[],"pid":{"revision_id":0,"type":"depid","value":"3679"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00003679","sets":["461:462"]},"author_link":["6270","6271","6272","6273"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"480","bibliographicPageStart":"477","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"JOURNAL OF VETERINARY MEDICAL SCIENCE"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):477-480 (2014)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Honda, Takayuki","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDUHFkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000333777400025"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"JAPAN SOC VET SCI"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"24285011"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/24285011","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1292/jvms.13-0357"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1292/jvms.13-0357","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright© 2014 The Japanese Society of Veterinary Science"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-7250","subitem_source_identifier_type":"PISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uehara,  Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuda,  Kazuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugano,  Mitsutoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honda,  Takayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-24"}],"displaytype":"detail","filename":"New_High-Speed_Droplet-Real-Time_Polymerase_Chain.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"New_High-Speed_Droplet-Real-Time_Polymerase_Chain.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/3679/files/New_High-Speed_Droplet-Real-Time_Polymerase_Chain.pdf"},"version_id":"0870ed4f-f0cb-4b5e-975e-b3a0ddcfe4b8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bovine respiratory syncytial virus","subitem_subject_scheme":"Other"},{"subitem_subject":"domestic animal","subitem_subject_scheme":"Other"},{"subitem_subject":"droplet-real-time PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"infectious disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-09-05"},"publish_date":"2014-09-05","publish_status":"0","recid":"3679","relation_version_is_last":true,"title":["A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:07:32.077603+00:00"}