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siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the A alpha-chain gene does not affect the plasma fibrinogen level
http://hdl.handle.net/10091/17325
http://hdl.handle.net/10091/1732563fc21ef-7e27-4a45-a094-1081a3a697fa
| 名前 / ファイル | ライセンス | アクション |
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siRNA_down-regulation_FGA_mRNA_HepG2_cells.pdf (372.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-12-27 | |||||
| タイトル | ||||||
| タイトル | siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the A alpha-chain gene does not affect the plasma fibrinogen level | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 関連識別子 | https://doi.org/10.1016/j.thromres.2013.01.010 | |||||
| 関連名称 | 10.1016/j.thromres.2013.01.010 | |||||
| キーワード | ||||||
| 主題 | afibrinogenemia, heterozygote, A alpha-chain, mRNA, siRNA, real-time quantitative RT-PCR | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Takezawa, Yuka
× Takezawa, Yuka× Matsuda, Kazuyuki× Terasawa, Fumiko× Sugano, Mitsutoshi× Honda, Takayuki× Okumura, Nobuo |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Honda, Takayuki | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDUHFkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Okumura, Nobuo | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html | |||||
| 出版者 | ||||||
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | THROMBOSIS RESEARCH. 131(4):342-348 (2013) | |||||
| 書誌情報 |
THROMBOSIS RESEARCH 巻 131, 号 4, p. 342-348, 発行日 2013-04 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: We encountered two afibrinogenemia patients with homozygous and compound heterozygous FGA mutation. Of interest, the patients' parents, who are heterozygous, had normal levels of plasma fibrinogen; thus, we hypothesized that liver FGA mRNA levels were higher than those of FGB and/or FGG mRNA. Materials and Methods: To test the hypothesis, we quantitated mRNA levels of a normal liver and a human hepatocyte cell line, HepG2 cells, and performed siRNA-mediated down-regulation of the fibrinogen gene in HepG2 cells. mRNA levels were determined using real-time quantitative RT-PCR for three normal livers and HepG2 cells. Down-regulation of FGA, FGB, or FGG in HepG2 cells was performed by the addition of siRNA corresponding to each of the three genes, and the mRNA levels determined in the cells and the secreted fibrinogen concentration in media. Results: The mRNA level of normal human liver was FGA=FGB>FGG and the FGG mRNA level was about 2-fold lower than the others, that of HepG2 cells was FGA>FGG>FGB and FGA mRNA was approximately 2- or 4-fold higher than FGG mRNA and FGB mRNA. When FGA, FGB, or FGG mRNA expression levels were down-regulated by nearby 50%, fibrinogen concentrations in media were 78%, 49%, or 57% of the control, respectively. Conclusions: Our results suggest that FGG mRNA levels limit fibrinogen expression in normal liver and HepG2 cells and that 50% reduction of FGA mRNA levels would not limit fibrinogen expression in normal liver and HepG2 cells. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0049-3848 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00863148 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/23414568 | |||||
| 関連名称 | 23414568 | |||||
| 権利 | ||||||
| 権利情報 | Copyright© 2013 Elsevier Ltd. | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000317234600025 | |||||
