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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the A alpha-chain gene does not affect the plasma fibrinogen level

http://hdl.handle.net/10091/17325
63fc21ef-7e27-4a45-a094-1081a3a697fa
名前 / ファイル ライセンス アクション
siRNA_down-regulation_FGA_mRNA_HepG2_cells.pdf siRNA_down-regulation_FGA_mRNA_HepG2_cells.pdf (372.4 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-12-27
タイトル
言語 en
タイトル siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the A alpha-chain gene does not affect the plasma fibrinogen level
言語
言語 eng
キーワード
主題Scheme Other
主題 afibrinogenemia
キーワード
主題Scheme Other
主題 heterozygote
キーワード
主題Scheme Other
主題 A alpha-chain
キーワード
主題Scheme Other
主題 mRNA
キーワード
主題Scheme Other
主題 siRNA
キーワード
主題Scheme Other
主題 real-time quantitative RT-PCR
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Takezawa, Yuka

× Takezawa, Yuka

WEKO 6955

en Takezawa, Yuka

Search repository
Matsuda, Kazuyuki

× Matsuda, Kazuyuki

WEKO 6956

en Matsuda, Kazuyuki

Search repository
Terasawa, Fumiko

× Terasawa, Fumiko

WEKO 6957

en Terasawa, Fumiko

Search repository
Sugano, Mitsutoshi

× Sugano, Mitsutoshi

WEKO 6958

en Sugano, Mitsutoshi

Search repository
Honda, Takayuki

× Honda, Takayuki

WEKO 6959

en Honda, Takayuki

Search repository
Okumura, Nobuo

× Okumura, Nobuo

WEKO 6960

en Okumura, Nobuo

Search repository
信州大学研究者総覧へのリンク
氏名 Honda, Takayuki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDUHFkh.html
信州大学研究者総覧へのリンク
氏名 Okumura, Nobuo
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html
出版者
出版者 PERGAMON-ELSEVIER SCIENCE LTD
引用
内容記述タイプ Other
内容記述 THROMBOSIS RESEARCH. 131(4):342-348 (2013)
書誌情報 THROMBOSIS RESEARCH

巻 131, 号 4, p. 342-348, 発行日 2013-04
抄録
内容記述タイプ Abstract
内容記述 Introduction: We encountered two afibrinogenemia patients with homozygous and compound heterozygous FGA mutation. Of interest, the patients' parents, who are heterozygous, had normal levels of plasma fibrinogen; thus, we hypothesized that liver FGA mRNA levels were higher than those of FGB and/or FGG mRNA. Materials and Methods: To test the hypothesis, we quantitated mRNA levels of a normal liver and a human hepatocyte cell line, HepG2 cells, and performed siRNA-mediated down-regulation of the fibrinogen gene in HepG2 cells. mRNA levels were determined using real-time quantitative RT-PCR for three normal livers and HepG2 cells. Down-regulation of FGA, FGB, or FGG in HepG2 cells was performed by the addition of siRNA corresponding to each of the three genes, and the mRNA levels determined in the cells and the secreted fibrinogen concentration in media. Results: The mRNA level of normal human liver was FGA=FGB>FGG and the FGG mRNA level was about 2-fold lower than the others, that of HepG2 cells was FGA>FGG>FGB and FGA mRNA was approximately 2- or 4-fold higher than FGG mRNA and FGB mRNA. When FGA, FGB, or FGG mRNA expression levels were down-regulated by nearby 50%, fibrinogen concentrations in media were 78%, 49%, or 57% of the control, respectively. Conclusions: Our results suggest that FGG mRNA levels limit fibrinogen expression in normal liver and HepG2 cells and that 50% reduction of FGA mRNA levels would not limit fibrinogen expression in normal liver and HepG2 cells.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 0049-3848
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00863148
PubMed
関連識別子
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/23414568
関連名称
関連名称 23414568
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.thromres.2013.01.010
関連名称
関連名称 10.1016/j.thromres.2013.01.010
権利
権利情報 Copyright© 2013 Elsevier Ltd.
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000317234600025
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