{"created":"2021-03-01T06:07:38.136161+00:00","id":3802,"links":{},"metadata":{"_buckets":{"deposit":"83f92592-857c-4e78-b8cc-124e5d87fd44"},"_deposit":{"id":"3802","owners":[],"pid":{"revision_id":0,"type":"depid","value":"3802"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00003802","sets":["461:462"]},"author_link":["7133","7134","7135","7136","7137","7138","7139"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"524","bibliographicPageStart":"516","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"CRYO LETTERS"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Poor adhesion of single human embryonic stem (hES) cells after freeze-thawing causes death. To investigate mechanisms responsible for this, Rho-dependent protein kinase (ROCK) inhibitor Y-27632-treated and untreated single hES cells were analyzed for E-cadherin and F-actin distribution by immunostaining and phalloidin staining respectively and for G13 signaling pathway components by DNA microarray and quantitative polymerase chain reaction (PCR). Y-27632-treated cells clustered rapidly and maintained E-cadherin and F-actin distribution without losing Oct3/4. Immediately after thawing, E-cadherin in untreated hES cells dotted along the membrane and then displayed eccentric cytoplasmic localization. Bleb formation and early Oct3/4 loss occurred after F-actin network condensation in the cytoplasm. Microarray analyses and quantitative PCR indicated upregulation of two actin reorganization-associated components of the G13 signaling pathway, Arhgdib and Cdc42, in untreated cells. Considering these findings and that cell death was partly interrupted by Y-27632, E-cadherin and actin cytoskeleton network disruption through the G13 signaling pathway may cause hES cell death after freeze-thawing.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"CRYO LETTERS. 32(6):516-524 (2011)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Tomotsune, Daihachiro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000298622900009"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"CRYO LETTERS"}]},"item_6_relation_46":{"attribute_name":"他の資源との関係：URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.cryoletters.org/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.cryoletters.org/","subitem_relation_type_select":"URI"}}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"22227712"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/22227712","subitem_relation_type_select":"PMID"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright© CryoLetters"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0143-2044","subitem_source_identifier_type":"PISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00145687","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ichikawa,  Hinako","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshie,  Susumu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirasawa,  Sakiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokoyama,  Tadayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yue,  Fengming","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomotsune,  Daihachiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki,  Katsunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-24"}],"displaytype":"detail","filename":"Cryoletters32.6.pp516-524.pdf","filesize":[{"value":"689.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Cryoletters32.6.pp516-524.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/3802/files/Cryoletters32.6.pp516-524.pdf"},"version_id":"a8185f9f-4133-4ce7-a575-90072b70af23"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cryopreservation","subitem_subject_scheme":"Other"},{"subitem_subject":"E-cadherin","subitem_subject_scheme":"Other"},{"subitem_subject":"actin filaments","subitem_subject_scheme":"Other"},{"subitem_subject":"G13 signaling pathway","subitem_subject_scheme":"Other"},{"subitem_subject":"human embryonic stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"ROCK inhibitor","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-04-01"},"publish_date":"2013-04-01","publish_status":"0","recid":"3802","relation_version_is_last":true,"title":["Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:12:15.138786+00:00"}