@article{oai:soar-ir.repo.nii.ac.jp:00003815, author = {Komatsu, Makiko and Tong, Yuhong and Li, Yufeng and Nakajima, Takero and Li, Gang and Hu, Rui and Sugiyama, Eiko and Kamijo, Yuji and Tanaka, Naoki and Hara, Atsushi and Aoyama, Toshifumi}, issue = {2}, journal = {GENES TO CELLS}, month = {Feb}, note = {Peroxisome proliferator-activated receptor alpha (PPAR alpha) is a member of the nuclear receptor family, regulating fatty acid degradation in many organs. Two-dimensional SDS-PAGE of brown adipose tissue (BAT) from PPAR alpha-null mice produced a higher-density spot. Proteomic analysis indicated that the protein was pyruvate dehydrogenase beta (PDH beta). To observe PDH beta regulation in BAT, the organ was stimulated by long-term cold exposure, and the activities of associated enzymes were investigated. Histological and biochemical analyses of BAT showed a significant decrease in the triglyceride content in wild-type mice and some degree of decrease in PPAR alpha-null mice on cold exposure. Analyses of molecules related to glucose metabolism showed that the expression of PDH beta is under PPAR alpha-specific regulation, and that glucose degradation ability may decrease on cold exposure. In contrast, analyses of molecules related to fatty acid metabolism showed that numerous PPAR alpha/gamma target molecules are induced on cold exposure, and that fatty acid degradation ability in wild-type mice is markedly enhanced and also increases to same degree in PPAR alpha-null mice on cold exposure. Thus, this study proposes novel and multiple roles of PPAR alpha in BAT., Article, GENES TO CELLS. 15(2):91-100 (2010)}, pages = {91--100}, title = {Multiple roles of PPAR alpha in brown adipose tissue under constitutive and cold conditions}, volume = {15}, year = {2010} }