{"created":"2021-03-01T06:07:39.683860+00:00","id":3828,"links":{},"metadata":{"_buckets":{"deposit":"3fc280ed-04b6-4859-a9d6-ab84bdb0f7ee"},"_deposit":{"id":"3828","owners":[],"pid":{"revision_id":0,"type":"depid","value":"3828"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00003828","sets":["461:462"]},"author_link":["7334","7335","7336","7337","7338","7339"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"583","bibliographicPageStart":"572","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lymphocyte homing is regulated by a multistep process mediated by sequential adhesive interactions between circulating lymphocytes and high endothelial venules (HEVs). In gut-associated lymphoid tissue (GALT), the initial interactive step, \"tethering and rolling,\" is partly mediated by integrin alpha 4 beta 7 expressed on GALT-homing lymphocytes and its ligand MAdCAM-1, which is exclusively expressed on HEVs in GALT. To probe functional MAdCAM-1 in tissue sections, we developed a soluble integrin alpha 4 beta 7 heterodimeric IgG chimera by joining the extracellular region of mouse integrin alpha 4 and beta 7 subunits to a human IgG Fc domain. Western blot analysis revealed that co-transfection of HEK 293T cells with expression vectors encoding integrin alpha 4.IgG and beta 7.IgG results in the formation of alpha 4 beta 7.IgG heterodimeric chimeras. This complex preferentially binds to CHO cells expressing MAdCAM-1 and, to a lesser extent, to cells expressing VCAM-1, but not to cells expressing ICAM-1. Moreover, alpha 4 beta 7.IgG specifically binds to HEVs in GALT in situ in a divalent cation-dependent fashion and inhibits lymphocyte binding to HEVs in GALT. These findings indicate that alpha 4 beta 7.IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration. (J Histochem Cytochem 59:572-583, 2011)","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 59(6):572-583 (2011)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Nakayama, Jun","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.HCApHFkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000293703900001"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SAGE PUBLICATIONS LTD"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"21430257"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/21430257","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1369/0022155411404416"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1369/0022155411404416","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-1554","subitem_source_identifier_type":"PISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA0069926X","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hoshino,  Hitomi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi,  Motohiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitoma,  Junya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato,  Yoshiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda,  Minoru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama,  Jun","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-24"}],"displaytype":"detail","filename":"An_Integrin_alpha_4_beta_7_IgG_Heterodimeric_Chimera.pdf","filesize":[{"value":"932.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"An_Integrin_alpha_4_beta_7_IgG_Heterodimeric_Chimera.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/3828/files/An_Integrin_alpha_4_beta_7_IgG_Heterodimeric_Chimera.pdf"},"version_id":"af94da54-3100-4753-9eb5-36e4a7309a9e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"alpha 4 beta 7 integrin","subitem_subject_scheme":"Other"},{"subitem_subject":"functional probe","subitem_subject_scheme":"Other"},{"subitem_subject":"gut-associated lymphoid tissue (GALT)","subitem_subject_scheme":"Other"},{"subitem_subject":"high endothelial venule (HEV)","subitem_subject_scheme":"Other"},{"subitem_subject":"lymphocyte homing","subitem_subject_scheme":"Other"},{"subitem_subject":"mucosal addressin cell adhesion molecule 1 (MAdCAM-1)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-02-04"},"publish_date":"2013-02-04","publish_status":"0","recid":"3828","relation_version_is_last":true,"title":["An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:12:03.010725+00:00"}