@article{oai:soar-ir.repo.nii.ac.jp:00003830,
 author = {Nakajima,  K and Takeoka,  M and Mori,  M and Hashimoto,  S and Sakurai,  A and Nose,  H and Higuchi,  K and Itano,  N and Shiohara,  M and Oh,  T and Taniguchi,  S},
 issue = {9},
 journal = {INTERNATIONAL JOURNAL OF SPORTS MEDICINE},
 month = {Sep},
 note = {Chronic moderate exercise has been reported to reduce pro-inflammatory cytokines. To analyze the molecular mechanisms by which training exerts these effects, the epigenetic influences of age and exercise on the ASC gene, which is responsible for IL-1 beta and IL-18 secretion, were investigated by ASC gene methylation. Further, the relationship between carcinogenesis and exercise, and methylation of the p15 tumor suppressive gene was also analyzed. High-intensity interval walking exercise, consisting of 3 min low-intensity walking at 40% of peak aerobic capacity followed by a 3 min high-intensity walking period above 70% of peak aerobic capacity, was continued for 6 months. Peripheral blood DNA extracts from young control (n = 34), older control (n = 153), and older exercise (n = 230) groups were then analyzed by pyrosequencing for DNA methylation. Methylation of ASC decreased significantly with age (young control vs. older control, p < 0.01), which is indicative of an age-dependent increase in ASC expression. Compared to the older control group, the degree of ASC methylation was higher in the older exercise group (older control vs. older exercise: p < 0.01), and presumably lower ASC expression. Neither exercise nor age affected the methylation of the p15. In summary, chronic moderate exercise appears to attenuate the age-dependent decrease in ASC methylation, implying suppression of excess pro-inflammatory cytokines through reduction of ASC expression., Article, INTERNATIONAL JOURNAL OF SPORTS MEDICINE. 31(9):671-675 (2010)},
 pages = {671--675},
 title = {Exercise Effects on Methylation of ASC Gene},
 volume = {31},
 year = {2010}
}