@article{oai:soar-ir.repo.nii.ac.jp:00003833, author = {Kuzumoto, K and Kubota, N and Ishii, K and Yumoto, K and Ogiso, Y and Nakamura, T and Katsuyama, T and Oana, K and Kawakami, Y}, issue = {12}, journal = {EUROPEAN JOURNAL OF MEDICAL RESEARCH}, month = {Dec}, note = {Background: Burkholderia cepacia strains have been known to possess the capability to cause serious infections especially in neonatal intensive care units (NICUs), and their multi-drug resistances become a severe threat in hospital settings. The aim of this investigation was to evaluate the B. cepacia complex infections in the NICU in Nagano Children's Hospital, Azumino 399-8288, Japan, and to report the intervention leading to the successful cessation of the outbreak. Methodology: The incidence of isolation and antimicrobial susceptibilities of nosocomial Burkholderia cepacia complex strains during a four-year period were retrospectively examined by clinical microbiological records, and by pulsed-field gel electrophoresis analyses along with the bacteriological verification of disinfectant device itself and procedures for its maintenance routinely used in the NICU. Results: During the period surveyed between 2007 and 2009, only an isolate per respective year of B. cepacia complex was recovered from each neonate in the NICU. However, in 2010, the successive 6 B. cepacia complex isolates were recovered from different hospitalized neonates. Among them, an isolate was originated from peripheral blood of a neonate, apparently giving rise to systemic infection. In addition, the hospitalized neonate with bacteremia due to B. cepacia complex also exhibited positive cultures from repeated catheterized urine samples together with tracheal aspirate secretions. However other 5 isolates were considered as the transients or contaminants having little to do with infections. Moreover, the 5 isolates between July and October in 2010 revealed completely the same electrophoresis patterns by means of pulsed-field gel electrophoresis analyses, strongly indicating that they were infected through the same medical practices, or by transmission of the same contaminant. Conclusions: A small outbreak due to B. cepacia comlex was brought about in the NICU in 2010, which appeared to be associated with the same genomovar of B. cepacia complex. The source or the rout of infection was unknown in spite of the repeated epidemiological investigation. It is noteworthy that no outbreak due to B. cepacia complex was noted in the NICU after extensive surveillance intervention., Article, EUROPEAN JOURNAL OF MEDICAL RESEARCH. 16(12):537-542 (2011)}, pages = {537--542}, title = {SUCCESSFUL CESSATION OF TRANSMITTING HEALTHCARE-ASSOCIATED INFECTIONS DUE TO BURKHOLDERIA CEPACIA COMPLEX IN A NEONATAL INTENSIVE CARE UNIT IN A JAPANESE CHILDREN'S HOSPITAL}, volume = {16}, year = {2011} }