{"created":"2021-03-01T06:08:00.077081+00:00","id":4169,"links":{},"metadata":{"_buckets":{"deposit":"8b5760b2-c802-422c-b546-8f5fd848406b"},"_deposit":{"id":"4169","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4169"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00004169","sets":["461:463:464:492"]},"author_link":["9460","9461","9462","9463","9464","9465","9466","9467","9468","9469","9470"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-02-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"26","bibliographicPageStart":"13","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"信州医学雑誌"}]}]},"item_10_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We analyzed Ca-permeating nonselective cation channels (NSCs)mediating thrombin-induced contraction of human umbilical vein endothelial cells (HUVECs). A Ca chelater, BAPTA-AM (10μM), significantly inhibited the thrombin-induced contraction of HUVECs.Thrombin induced inward currents at -60 mV in the presence of intracellular MgATP. Removal of extracellular Caﾌﾞｸﾞsignificantly decreased the currents. A selective phospholipase C inhibitor, U73122 (1μM) but not its inactive analogue, U73343 (1μM) almost completely inhibited the currents. Neither a selective inhibitor of Caﾌﾞｸﾞ-ATPase of endoplasmic reticulum, thapsigargin (1μM)nor a diacylglycerol analogue, 1-oleoyl-2-acetyl-glycerol (30μM)activated the currents. However, a selective protein kinase C inhibitor, bisindolylmaleimide I (500 nM) significantly inhibited the currents.The thrombin-induced currents were significantly inhibited by SKF96365 (50μM)but not by La(1mM), ruthenium red (10μM) or flufenamic acid (100μM). As assessed with RT-PCR, HUVECs expressed transient receptor potential(TRP)M4,7,TRPV1,2,4,TRPC1,4 and 6 subunits of NSCs.These results indicate that thrombin activates Ca-permeating NSCs containing TRPC4 through protein kinase C in HUVECs. Thus,drugs specifically inhibiting TRPC4-containing channels might be effective to control fatal diseases such as sepsis where thrombin mediates the vicious cycle between inflammation and coagulation.","subitem_description_type":"Abstract"}]},"item_10_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"信州医学雑誌 59(1): 13-26(2011)","subitem_description_type":"Other"}]},"item_10_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"OHHASHI, Toshio","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.HFApOFkh.html"},{"subitem_link_text":"YAMADA, Mitsuhiko","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.jhSCupca.html"}]},"item_10_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州医学会"}]},"item_10_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.11441/shinshumedj.59.13"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11441/shinshumedj.59.13","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0037-3826","subitem_source_identifier_type":"PISSN"}]},"item_10_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00120815","subitem_source_identifier_type":"NCID"}]},"item_1627890569677":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TSUJINO, Natsuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKADA, Tsutomu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUBOUCHI, Kiyotaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KOBAYASHI, Masaaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAWAI, Yoshiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YANO, Shiharu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ATSUNAGA, Tamihide","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIROSE, Masamichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHMORI, Shigeru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHHASHI,  Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMADA,  Mitsuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-24"}],"displaytype":"detail","filename":"Shinshu_med59-1-02.pdf","filesize":[{"value":"991.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Shinshu_med59-1-02.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/4169/files/Shinshu_med59-1-02.pdf"},"version_id":"59265691-bb54-408c-a8c9-5c29af41f41c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"human umbilical vein endothelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thrombin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nonselective cation channels","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transient receptor potential C4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein kinase C","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["492"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-02-15"},"publish_date":"2011-02-15","publish_status":"0","recid":"4169","relation_version_is_last":true,"title":["Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-03-06T01:37:43.894672+00:00"}