@article{oai:soar-ir.repo.nii.ac.jp:00004373,
 author = {矢嶋,  紀幸 and 鈴木,  淳一 and 北林,  浩 and 磯部,  光章},
 issue = {5},
 journal = {信州医学雑誌},
 month = {Oct},
 note = {Myocarditis is a potentially lethal inflammatory disease in humans.In this disease,large amounts of cytokines are released from inflammatory cells,which then stimulate endothelial cells to secrete endothelin-1 (ET-1). ET-1 has been isolated from endothelial cells and is a key mediator in several cardiovascular diseases. However, the immunological effects of ET-1 in myocarditis have not been reported.To investigate the roles of ET-1 expression in myocarditis, immunohistochemistry and reverse transcription-polymerase chain eaction (RT-PCR)were used to detect ET-1 in rat hearts with experimental autoimmune myocarditis (EAM). The EAM rat hearts were harvested at days 7,14,21 and 28 (n＝12).For the control study,hearts from rats without immunization were also harvested at day 28 (n＝5). Immunohistochemically, ET-1 was faintly expressed in unimmunized hearts.In EAM hearts,ET-1 expression was enhanced in the endothelial cells of small vasculatures in the myocardium at days 7 and 14.ET-1 was observed prior to interstitial cell infiltration and myocardial damage. However, ET-1 expression was decreased in the myocardium at days 21 and 28. Using RT-PCR,a single band corresponding to ET-1 was detected in unimmunized and EAM samples on days 7 to 28.In this study,ET-1 was enhanced in the early phase of EAM,and decreased in the chronic phase.ET-1 may therefore play a critical role in the early stage of EAM inflammation and may have diagnostic value in clinical myocarditis., Article, 信州医学雑誌 47(5): 389-391(1999)},
 pages = {389--391},
 title = {ラット自己免疫性心筋炎モデル心内におけるエンドセリン-１の発現},
 volume = {47},
 year = {1999}
}